1M04

Mutant Streptomyces plicatus beta-hexosaminidase (D313N) in complex with product (GlcNAc)

Summary for 1M04

Entry DOI	10.2210/pdb1m04/pdb
Related	1M01 1M03 1hp5 1jak
Descriptor	Beta-N-acetylhexosaminidase, 2-acetamido-2-deoxy-beta-D-glucopyranose, CHLORIDE ION, ... (6 entities in total)
Functional Keywords	substrate assisted catalysis, hexosaminidase, family 20 glycosidase, hydrolase
Biological source	Streptomyces plicatus
Total number of polymer chains	1
Total formula weight	56641.52
Authors	Williams, S.J.,Mark, B.L.,Vocadlo, D.J.,James, M.N.G.,Withers, S.G. (deposition date: 2002-06-11, release date: 2002-12-11, Last modification date: 2021-10-27)
Primary citation	Williams, S.J.,Mark, B.L.,Vocadlo, D.J.,James, M.N.,Withers, S.G. Aspartate 313 in the Streptomyces plicatus hexosaminidase plays a critical role in substrate-assisted catalysis by orienting the 2-acetamido group and stabilizing the transition state. J.Biol.Chem., 277:40055-40065, 2002 Cited by PubMed: 12171933 DOI: 10.1074/jbc.M206481200 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.95 Å)

Structure validation