12DL
Native structure of the cytoplasmic lattice (CPL) asymmetric unit from mouse MII eggs
This is a non-PDB format compatible entry.
Summary for 12DL
| Entry DOI | 10.2210/pdb12dl/pdb |
| EMDB information | 76334 |
| Descriptor | Tubulin beta-4B chain, NACHT, LRR and PYD domains-containing protein 14, E3 ubiquitin-protein ligase UHRF1, ... (20 entities in total) |
| Functional Keywords | cytoplasmic lattice, egg, filamentous assembly, cytosolic protein |
| Biological source | Mus musculus (house mouse) More |
| Total number of polymer chains | 33 |
| Total formula weight | 2137881.17 |
| Authors | |
| Primary citation | Li, Y.,Zheng, W.,Leem, J.,Wu, C.,Tang, S.,Mogessie, B.,Xiong, Y. Cytoplasmic lattices store developmentally poised degradative and cytoskeletal complexes in mammalian eggs. Nat.Struct.Mol.Biol., 2026 Cited by PubMed Abstract: The cytoplasmic lattice (CPL) in mammalian eggs is essential for early embryonic development but its molecular components, structural organization and functional capacity have remained elusive. Here, using cryo-electron microscopy, we show that the CPL filament in mouse metaphase II eggs contains repeating units with a periodicity of ~37 nm and determine its high-resolution, native structure and complete subunit composition. The CPL architecture organizes maternal-effect proteins, ubiquitination machinery and tubulin into a highly structured reservoir. Maternal-effect proteins form the scaffold of the CPL to sequester a UHRF1-UBE2D3 E3-E2 ubiquitination module and three distinct FBXW-SKP1 E3 ubiquitin ligase components, notably all in activity-excluded states. The CPL further contains αβ-tubulin heterodimers in a GTP-bound state, indicating microtubule-assembly-competent tubulin held in reserve. CPL filaments are capped by a terminal unit that lacks a PADI6 dimer, a scaffold component, suggesting a structural mechanism that prevents further oligomerization. Interactions between neighboring CPL filaments promote the assembly of a three-dimensional network in the egg cytoplasm. Taken together, our work defines how CPL assembly and architecture prime mammalian eggs for ubiquitin-mediated protein degradation and cytoskeletal remodeling during the egg-to-embryo transition. PubMed: 42426369DOI: 10.1038/s41594-026-01843-2 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.5 Å) |
Structure validation
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