9ZXC

Structure of AT118-R nanobody in complex with the angiotensin II type I receptor bound to losartan

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Nanobody AT118-R,Type-1 angiotensin II receptor with Soluble cytochrome b562 insertion	polymer	582	65389.8	1	UniProt (P30556) UniProt (P0ABE7)	synthetic construct
2	B (C)	BAG2 Anti-BRIL Fab Heavy Chain	polymer	231	24539.3	1		synthetic construct
3	C (D)	BAG2 Anti-BRIL Fab Light Chain	polymer	215	23541.2	1		synthetic construct
4	D, E (A)	CHOLESTEROL HEMISUCCINATE	non-polymer		486.7	2	Chemie (Y01)
5	F (A)	[2-butyl-5-chloranyl-3-[[4-[2-(2H-1,2,3,4-tetrazol-5-yl)phenyl]phenyl]methyl]imidazol-4-yl]methanol	non-polymer		422.9	1	Chemie (LSN)

Sequence modifications

A: -134 - 1 (PDB: 9ZXC)
A: 2 - 226 (UniProt: P30556)
A: 227 - 330 (UniProt: P0ABE7)

PDB	External Database	Details
Trp 232	Met 29	engineered mutation
Ile 327	His 124	engineered mutation
Leu 331	-	linker
Glu 332	-	linker
Arg 333	-	linker
Ala 334	-	linker
Arg 335	-	linker
Ser 336	-	linker
Thr 337	-	linker
Leu 338	-	linker
Asp 339	-	linker
Lys 340	-	linker
Leu 341	-	linker

A: 1235 - 1319 (UniProt: P30556)

PDB	External Database	Details
Gly 1320	-	expression tag
Gly 1321	-	expression tag
Ser 1322	-	expression tag
Ser 1323	-	expression tag
Leu 1324	-	expression tag
Glu 1325	-	expression tag
Val 1326	-	expression tag
Leu 1327	-	expression tag
Phe 1328	-	expression tag
Gln 1329	-	expression tag
Gly 1330	-	expression tag
Pro 1331	-	expression tag
Thr 1332	-	expression tag
Glu 1333	-	expression tag
Thr 1334	-	expression tag
Ser 1335	-	expression tag
Gln 1336	-	expression tag
Val 1337	-	expression tag
Ala 1338	-	expression tag
Pro 1339	-	expression tag
Ala 1340	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	113470.3
Non-Polymers*	Number of molecules	3
	Total formula weight	1396.4
All*	Total formula weight	114866.7

*Water molecules are not included.