9Z0E

Structure of disulfide-crosslinked S. cerevisiae Hrd1 dimer bound to one copy of Hrd3 in MSP1D1 nanodisc

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (B, C)	ERAD-associated E3 ubiquitin-protein ligase HRD1	polymer	344	40129.1	2	UniProt (Q08109) Pfam (PF25563)	Saccharomyces cerevisiae (brewer's yeast)	HMG-CoA reductase degradation protein 1,RING-type E3 ubiquitin transferase HRD1
2	C (A)	ERAD-associated E3 ubiquitin-protein ligase component HRD3	polymer	818	93787.2	1	UniProt (Q05787) Pfam (PF08238)	Saccharomyces cerevisiae (brewer's yeast)	HMG-CoA reductase degradation protein 3

Sequence modifications

B, C: 1 - 326 (UniProt: Q08109)

PDB	External Database	Details
Cys 91	Ile 91	engineered mutation
Gly 327	-	expression tag
Ser 328	-	expression tag
Gly 329	-	expression tag
Gly 330	-	expression tag
Ala 331	-	expression tag
Ser 332	-	expression tag
Gly 333	-	expression tag
Gly 334	-	expression tag
Ser 335	-	expression tag
Gly 336	-	expression tag
Asp 337	-	expression tag
Tyr 338	-	expression tag
Lys 339	-	expression tag
Asp 340	-	expression tag
Asp 341	-	expression tag
Asp 342	-	expression tag
Asp 343	-	expression tag
Lys 344	-	expression tag

A: 1 - 767 (UniProt: Q05787)

PDB	External Database	Details
Gly 768	-	expression tag
Gly 769	-	expression tag
Gly 770	-	expression tag
Glu 771	-	expression tag
Asn 772	-	expression tag
Leu 773	-	expression tag
Tyr 774	-	expression tag
Phe 775	-	expression tag
Gln 776	-	expression tag
Ser 777	-	expression tag
Gly 778	-	expression tag
Gly 779	-	expression tag
Gly 780	-	expression tag
Met 781	-	expression tag
Asp 782	-	expression tag
Glu 783	-	expression tag
Arg 784	-	expression tag
Thr 785	-	expression tag
Thr 786	-	expression tag
Gly 787	-	expression tag
Trp 788	-	expression tag
Arg 789	-	expression tag
Gly 790	-	expression tag
Gly 791	-	expression tag
His 792	-	expression tag
Val 793	-	expression tag
Val 794	-	expression tag
Glu 795	-	expression tag
Gly 796	-	expression tag
Leu 797	-	expression tag
Ala 798	-	expression tag
Gly 799	-	expression tag
Glu 800	-	expression tag
Leu 801	-	expression tag
Glu 802	-	expression tag
Gln 803	-	expression tag
Leu 804	-	expression tag
Arg 805	-	expression tag
Ala 806	-	expression tag
Arg 807	-	expression tag
Leu 808	-	expression tag
Glu 809	-	expression tag
His 810	-	expression tag
His 811	-	expression tag
Pro 812	-	expression tag
Gln 813	-	expression tag
Gly 814	-	expression tag
Gln 815	-	expression tag
Arg 816	-	expression tag
Glu 817	-	expression tag
Pro 818	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	174045.5
All*	Total formula weight	174045.5

*Water molecules are not included.