9Z0E
Structure of disulfide-crosslinked S. cerevisiae Hrd1 dimer bound to one copy of Hrd3 in MSP1D1 nanodisc
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 BIOCONTINUUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.7 |
| Software | cryoSPARC,SerialEM,Coot,PHENIX |
| CTF correction | NONE |
