9XZJ
Cryo-EM structure of F-box helicase 1 (FBH1) bound to an SCF ubiquitin ligase complex and a 3-way DNA fork (consensus structure)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (C) | Cullin-1 | polymer | 776 | 89800.3 | 1 | UniProt (Q13616) | Homo sapiens (human) | CUL-1 |
| 2 | B (R) | E3 ubiquitin-protein ligase RBX1 | polymer | 108 | 12290.0 | 1 | UniProt (P62877) | Homo sapiens (human) | E3 ubiquitin-protein transferase RBX1,Protein ZYP,RING finger protein 75,RING-box protein 1,Rbx1,Regulator of cullins 1,ROC1 |
| 3 | C (F) | F-box DNA helicase 1 | polymer | 972 | 109518.7 | 1 | UniProt (Q8NFZ0) | Homo sapiens (human) | hFBH1,DNA 3'-5' helicase 1,F-box only protein 18 |
| 4 | D (S) | S-phase kinase-associated protein 1 | polymer | 163 | 18680.0 | 1 | UniProt (P63208) | Homo sapiens (human) | SKP1,Cyclin-A/CDK2-associated protein p19,p19A,Organ of Corti protein 2,OCP-2,Organ of Corti protein II,OCP-II,RNA polymerase II elongation factor-like protein,SIII,Transcription elongation factor B polypeptide 1-like,p19skp1 |
| 5 | E (X) | DNA (45-MER) | polymer | 45 | 13921.9 | 1 | synthetic construct | ||
| 6 | F (A) | DNA (5'-D(*CP*TP*GP*AP*CP*GP*CP*TP*TP*CP*CP*AP*TP*CP*GP*CP*TP*GP*TP*CP*TP*AP*G)-3') | polymer | 23 | 6983.5 | 1 | synthetic construct | ||
| 7 | G (B) | DNA (5'-D(*TP*CP*GP*CP*TP*AP*CP*CP*TP*TP*CP*GP*CP*AP*GP*TP*C)-3') | polymer | 17 | 5114.3 | 1 | synthetic construct | ||
| 8 | H (Y) | DNA (45-MER) | polymer | 45 | 13872.9 | 1 | synthetic construct | ||
| 9 | I (F) | PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER | non-polymer | 523.2 | 1 | Chemie (AGS) | |||
| 10 | J (F) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 11 | K (F) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) |
Sequence modifications
C: 1 - 776 (UniProt: Q13616)
F: 1 - 969 (UniProt: Q8NFZ0)
| PDB | External Database | Details |
|---|---|---|
| Glu 689 | Lys 689 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ala 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 270181.6 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 613.0 | |
| All* | Total formula weight | 270794.6 |
