9TI8
Staphylococcus aureus 50S ribosome in complex with RRF, EF-G and fusidic acid (50S-RRF-EF-G-FA)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (1) | 50S ribosomal protein L28 | polymer | 62 | 6995.3 | 1 | UniProt (Q2FZ60) Pfam (PF00830) | Staphylococcus aureus subsp. aureus NCTC 8325 | |
| 2 | B (2) | 50S ribosomal protein L29 | polymer | 69 | 8105.3 | 1 | UniProt (Q2FW14) Pfam (PF00831) | Staphylococcus aureus subsp. aureus NCTC 8325 | |
| 3 | C (3) | 50S ribosomal protein L30 | polymer | 59 | 6565.7 | 1 | UniProt (P0A0G2) Pfam (PF00327) | Staphylococcus aureus subsp. aureus NCTC 8325 | |
| 4 | D (4) | 50S ribosomal protein L31 type B | polymer | 84 | 9737.9 | 1 | UniProt (Q2FWD8) Pfam (PF01197) | Staphylococcus aureus subsp. aureus NCTC 8325 | |
| 5 | E (5) | Large ribosomal subunit protein bL32 | polymer | 57 | 6500.6 | 1 | UniProt (Q2FZF1) Pfam (PF01783) | Staphylococcus aureus subsp. aureus NCTC 8325 | 50S ribosomal protein L32 |
| 6 | F (6) | Large ribosomal subunit protein bL33A | polymer | 49 | 5944.9 | 1 | UniProt (Q2FYU6) Pfam (PF00471) | Staphylococcus aureus subsp. aureus NCTC 8325 | 50S ribosomal protein L33 1 |
| 7 | G (7) | 50S ribosomal protein L34 | polymer | 45 | 5454.6 | 1 | UniProt (Q2FUQ0) Pfam (PF00468) | Staphylococcus aureus subsp. aureus NCTC 8325 | |
| 8 | H (8) | 50S ribosomal protein L35 | polymer | 66 | 7722.4 | 1 | UniProt (Q2FXQ0) Pfam (PF01632) | Staphylococcus aureus subsp. aureus NCTC 8325 | |
| 9 | I (9) | 50S ribosomal protein L36 | polymer | 37 | 4318.4 | 1 | UniProt (Q2FW29) Pfam (PF00444) | Staphylococcus aureus subsp. aureus NCTC 8325 | |
| 10 | J (A) | 23S rRNA | polymer | 2923 | 946811.7 | 1 | Staphylococcus aureus subsp. aureus NCTC 8325 | ||
| 11 | K (B) | 5S rRNA | polymer | 115 | 36974.9 | 1 | GenBank (CP000253.1) | Staphylococcus aureus subsp. aureus NCTC 8325 | |
| 12 | L (C) | Ribosome-recycling factor | polymer | 186 | 20576.2 | 1 | UniProt (Q2FZ21) Pfam (PF01765) | Staphylococcus aureus subsp. aureus NCTC 8325 | RRF,Ribosome-releasing factor |
| 13 | M (E) | Elongation factor G | polymer | 693 | 76699.3 | 1 | UniProt (Q2G0N1) Pfam (PF00009) Pfam (PF03144) Pfam (PF14492) Pfam (PF03764) Pfam (PF00679) | Staphylococcus aureus subsp. aureus NCTC 8325 | EF-G |
| 14 | N (G) | 50S ribosomal protein L2 | polymer | 277 | 30217.2 | 1 | UniProt (P60430) Pfam (PF00181) Pfam (PF03947) | Staphylococcus aureus subsp. aureus NCTC 8325 | |
| 15 | O (H) | 50S ribosomal protein L3 | polymer | 220 | 23760.3 | 1 | UniProt (Q2FW06) Pfam (PF00297) | Staphylococcus aureus subsp. aureus NCTC 8325 | |
| 16 | P (I) | 50S ribosomal protein L4 | polymer | 207 | 22495.7 | 1 | UniProt (Q2FW07) Pfam (PF00573) | Staphylococcus aureus subsp. aureus NCTC 8325 | |
| 17 | Q (J) | 50S ribosomal protein L5 | polymer | 179 | 20296.6 | 1 | UniProt (Q2FW18) Pfam (PF00281) Pfam (PF00673) | Staphylococcus aureus subsp. aureus NCTC 8325 | |
| 18 | R (K) | Large ribosomal subunit protein uL6 | polymer | 178 | 19818.6 | 1 | UniProt (Q2FW21) Pfam (PF00347) | Staphylococcus aureus subsp. aureus NCTC 8325 | 50S ribosomal protein L6 |
| 19 | S (M) | 50S ribosomal protein L13 | polymer | 145 | 16359.4 | 1 | UniProt (Q2FW38) Pfam (PF00572) | Staphylococcus aureus subsp. aureus NCTC 8325 | |
| 20 | T (N) | 50S ribosomal protein L14 | polymer | 122 | 13157.3 | 1 | UniProt (Q2FW16) Pfam (PF00238) | Staphylococcus aureus subsp. aureus NCTC 8325 | |
| 21 | U (O) | 50S ribosomal protein L15 | polymer | 146 | 15628.9 | 1 | UniProt (P0A0F8) Pfam (PF00828) | Staphylococcus aureus subsp. aureus NCTC 8325 | |
| 22 | V (P) | 50S ribosomal protein L16 | polymer | 144 | 16274.0 | 1 | UniProt (Q2FW13) Pfam (PF00252) | Staphylococcus aureus subsp. aureus NCTC 8325 | |
| 23 | W (Q) | 50S ribosomal protein L17 | polymer | 122 | 13771.8 | 1 | UniProt (Q2FW33) Pfam (PF01196) | Staphylococcus aureus subsp. aureus NCTC 8325 | |
| 24 | X (R) | 50S ribosomal protein L18 | polymer | 119 | 13124.1 | 1 | UniProt (Q2FW22) Pfam (PF00861) | Staphylococcus aureus subsp. aureus NCTC 8325 | |
| 25 | Y (S) | 50S ribosomal protein L19 | polymer | 116 | 13392.8 | 1 | UniProt (Q2FZ42) Pfam (PF01245) | Staphylococcus aureus subsp. aureus NCTC 8325 | |
| 26 | Z (T) | 50S ribosomal protein L20 | polymer | 118 | 13720.3 | 1 | UniProt (Q2FXQ1) Pfam (PF00453) | Staphylococcus aureus subsp. aureus NCTC 8325 | |
| 27 | AA (U) | 50S ribosomal protein L21 | polymer | 102 | 11354.1 | 1 | UniProt (Q2FXS8) Pfam (PF00829) | Staphylococcus aureus subsp. aureus NCTC 8325 | |
| 28 | BA (V) | 50S ribosomal protein L22 | polymer | 117 | 12857.9 | 1 | UniProt (Q2FW11) Pfam (PF00237) | Staphylococcus aureus subsp. aureus NCTC 8325 | |
| 29 | CA (W) | 50S ribosomal protein L23 | polymer | 91 | 10625.4 | 1 | UniProt (Q2FW08) Pfam (PF00276) | Staphylococcus aureus subsp. aureus NCTC 8325 | |
| 30 | DA (X) | 50S ribosomal protein L24 | polymer | 105 | 11561.5 | 1 | UniProt (Q2FW17) Pfam (PF00467) Pfam (PF17136) | Staphylococcus aureus subsp. aureus NCTC 8325 | |
| 31 | EA (Y) | 50S ribosomal protein L25 | polymer | 217 | 23810.6 | 1 | UniProt (Q2G0S0) Pfam (PF01386) Pfam (PF14693) | Staphylococcus aureus subsp. aureus NCTC 8325 | General stress protein CTC |
| 32 | FA (Z) | 50S ribosomal protein L27 | polymer | 94 | 10334.8 | 1 | UniProt (Q2FXT0) Pfam (PF01016) | Staphylococcus aureus subsp. aureus NCTC 8325 | |
| 33 | GA, HA (5, 9) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) PubChem (32051) | |||
| 34 | AB, AC, AD, AE, BB... (A, E, G, H) | MAGNESIUM ION | non-polymer | 24.3 | 115 | Chemie (MG) PubChem (888) | |||
| 35 | QE (E) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) | |||
| 36 | SE (E) | FUSIDIC ACID | non-polymer | 516.7 | 1 | Chemie (FUA) PubChem (3000226) |
Sequence modifications
5: 1 - 57 (UniProt: Q2FZF1)
C: 1 - 184 (UniProt: Q2FZ21)
| PDB | External Database | Details |
|---|---|---|
| - | Arg 35 | deletion |
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| His -1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 32 |
| Total formula weight | 1454968.5 | |
| Non-Polymers* | Number of molecules | 119 |
| Total formula weight | 3885.8 | |
| All* | Total formula weight | 1458854.3 |
