9TI8
Staphylococcus aureus 50S ribosome in complex with RRF, EF-G and fusidic acid (50S-RRF-EF-G-FA)
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.18 |
| Number of Particle | 2556350 |
| Software | cryoSPARC,Servalcat,EPU,Coot |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
