9SH5
The cryo-EM structure of human Tissue Nonspecific Alkaline Phosphatase (hTNAP) in complex with MLS-0038949
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Alkaline phosphatase, tissue-nonspecific isozyme | polymer | 512 | 56739.3 | 2 | UniProt (P05186) Pfam (PF00245) | Homo sapiens (human) | AP-TNAP,TNS-ALP,TNSALP,Alkaline phosphatase liver/bone/kidney isozyme,Phosphoamidase,Phosphocreatine phosphatase |
| 2 | C, L (C, L) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 2 | In PDB GlyTouCan (G15407YE) | |||
| 3 | D, E, I, K (D, E, I, K) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 4 | In PDB GlyTouCan (G42666HT) | |||
| 4 | F, J (F, J) | alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 367.3 | 2 | In PDB GlyTouCan (G86851RC) | |||
| 5 | G, H (G, H) | alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 894.8 | 2 | In PDB GlyTouCan (G42466VF) | |||
| 6 | M, R (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 7 | N, P, S, U (A, B) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) | |||
| 8 | O, T (A, B) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 9 | Q, V (A, B) | 2,5-dimethoxy-~{N}-quinolin-3-yl-benzenesulfonamide | non-polymer | 344.4 | 2 | Chemie (A1JNY) |
Sequence modifications
A, B: 18 - 500 (UniProt: P05186)
| PDB | External Database | Details |
|---|---|---|
| Gly 15 | - | expression tag |
| Arg 16 | - | expression tag |
| Ala 17 | - | expression tag |
| Ala 501 | - | expression tag |
| Ala 502 | - | expression tag |
| Ala 503 | - | expression tag |
| Glu 504 | - | expression tag |
| Asn 505 | - | expression tag |
| Leu 506 | - | expression tag |
| Tyr 507 | - | expression tag |
| Phe 508 | - | expression tag |
| Gln 509 | - | expression tag |
| Gly 510 | - | expression tag |
| Asp 511 | - | expression tag |
| Tyr 512 | - | expression tag |
| Lys 513 | - | expression tag |
| Asp 514 | - | expression tag |
| Asp 515 | - | expression tag |
| Asp 516 | - | expression tag |
| Asp 517 | - | expression tag |
| Lys 518 | - | expression tag |
| His 519 | - | expression tag |
| His 520 | - | expression tag |
| His 521 | - | expression tag |
| His 522 | - | expression tag |
| His 523 | - | expression tag |
| His 524 | - | expression tag |
| His 525 | - | expression tag |
| His 526 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 113478.6 | |
| Branched | Number of molecules | 10 |
| Total formula weight | 5395.0 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 1079.2 | |
| All* | Total formula weight | 119952.8 |
