9SH5

The cryo-EM structure of human Tissue Nonspecific Alkaline Phosphatase (hTNAP) in complex with MLS-0038949

This is a non-PDB format compatible entry.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001501	biological_process	skeletal system development
A	0001649	biological_process	osteoblast differentiation
A	0004035	molecular_function	alkaline phosphatase activity
A	0004427	molecular_function	inorganic diphosphate phosphatase activity
A	0005509	molecular_function	calcium ion binding
A	0005576	cellular_component	extracellular region
A	0005615	cellular_component	extracellular space
A	0005758	cellular_component	mitochondrial intermembrane space
A	0005886	cellular_component	plasma membrane
A	0016020	cellular_component	membrane
A	0016462	molecular_function	pyrophosphatase activity
A	0016787	molecular_function	hydrolase activity
A	0016887	molecular_function	ATP hydrolysis activity
A	0030282	biological_process	bone mineralization
A	0031012	cellular_component	extracellular matrix
A	0031214	biological_process	biomineral tissue development
A	0031966	cellular_component	mitochondrial membrane
A	0032496	biological_process	response to lipopolysaccharide
A	0032868	biological_process	response to insulin
A	0033280	biological_process	response to vitamin D
A	0033883	molecular_function	pyridoxal phosphatase activity
A	0034516	biological_process	response to vitamin B6
A	0036005	biological_process	response to macrophage colony-stimulating factor
A	0042822	biological_process	pyridoxal phosphate metabolic process
A	0043262	molecular_function	ADP phosphatase activity
A	0046872	molecular_function	metal ion binding
A	0050187	molecular_function	phosphoamidase activity
A	0051384	biological_process	response to glucocorticoid
A	0052732	molecular_function	phosphoethanolamine phosphatase activity
A	0055062	biological_process	phosphate ion homeostasis
A	0055074	biological_process	calcium ion homeostasis
A	0065010	cellular_component	extracellular membrane-bounded organelle
A	0070062	cellular_component	extracellular exosome
A	0071529	biological_process	cementum mineralization
A	0098552	cellular_component	side of membrane
A	0120162	biological_process	positive regulation of cold-induced thermogenesis
A	0140651	biological_process	futile creatine cycle
A	0140928	biological_process	inhibition of non-skeletal tissue mineralization
A	1904383	biological_process	response to sodium phosphate
B	0001501	biological_process	skeletal system development
B	0001649	biological_process	osteoblast differentiation
B	0004035	molecular_function	alkaline phosphatase activity
B	0004427	molecular_function	inorganic diphosphate phosphatase activity
B	0005509	molecular_function	calcium ion binding
B	0005576	cellular_component	extracellular region
B	0005615	cellular_component	extracellular space
B	0005758	cellular_component	mitochondrial intermembrane space
B	0005886	cellular_component	plasma membrane
B	0016020	cellular_component	membrane
B	0016462	molecular_function	pyrophosphatase activity
B	0016787	molecular_function	hydrolase activity
B	0016887	molecular_function	ATP hydrolysis activity
B	0030282	biological_process	bone mineralization
B	0031012	cellular_component	extracellular matrix
B	0031214	biological_process	biomineral tissue development
B	0031966	cellular_component	mitochondrial membrane
B	0032496	biological_process	response to lipopolysaccharide
B	0032868	biological_process	response to insulin
B	0033280	biological_process	response to vitamin D
B	0033883	molecular_function	pyridoxal phosphatase activity
B	0034516	biological_process	response to vitamin B6
B	0036005	biological_process	response to macrophage colony-stimulating factor
B	0042822	biological_process	pyridoxal phosphate metabolic process
B	0043262	molecular_function	ADP phosphatase activity
B	0046872	molecular_function	metal ion binding
B	0050187	molecular_function	phosphoamidase activity
B	0051384	biological_process	response to glucocorticoid
B	0052732	molecular_function	phosphoethanolamine phosphatase activity
B	0055062	biological_process	phosphate ion homeostasis
B	0055074	biological_process	calcium ion homeostasis
B	0065010	cellular_component	extracellular membrane-bounded organelle
B	0070062	cellular_component	extracellular exosome
B	0071529	biological_process	cementum mineralization
B	0098552	cellular_component	side of membrane
B	0120162	biological_process	positive regulation of cold-induced thermogenesis
B	0140651	biological_process	futile creatine cycle
B	0140928	biological_process	inhibition of non-skeletal tissue mineralization
B	1904383	biological_process	response to sodium phosphate

Functional Information from PROSITE/UniProt

site_id	PS00123
Number of Residues	9
Details	ALKALINE_PHOSPHATASE Alkaline phosphatase active site. VpDSAGTAT

Chain	Residue	Details
A	VAL107-THR115

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Active site: {"description":"Phosphoserine intermediate","evidences":[{"source":"UniProtKB","id":"P05187","evidenceCode":"ECO:0000250"},{"source":"PROSITE-ProRule","id":"PRU10042","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	18
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P05187","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11395499","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P09242","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	8
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"19159218","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---