9RWZ

ZSWIM8-CUL3 complex bound to AGO2-miR-7-CYRANO

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Protein argonaute-2	polymer	870	98554.4	1	UniProt (Q9UKV8)	Homo sapiens (human)	Argonaute2,hAgo2,Argonaute RISC catalytic component 2,Eukaryotic translation initiation factor 2C 2,eIF-2C 2,eIF2C 2,PAZ Piwi domain protein,PPD,Protein slicer
2	B, C (B, C)	Elongin-B	polymer	118	13147.8	2	UniProt (Q15370)	Homo sapiens (human)	EloB,Elongin 18 kDa subunit,RNA polymerase II transcription factor SIII subunit B,SIII p18,Transcription elongation factor B polypeptide 2
3	D, E (D, E)	Elongin-C	polymer	112	12485.1	2	UniProt (Q15369)	Homo sapiens (human)	EloC,Elongin 15 kDa subunit,RNA polymerase II transcription factor SIII subunit C,SIII p15,Transcription elongation factor B polypeptide 1
4	F (G)	miR-7	polymer	24	7690.5	1		Homo sapiens
5	G, H (H, I)	Cullin-3	polymer	396	46410.1	2	UniProt (Q13618)	Homo sapiens (human)	CUL-3
6	I (T)	CYRANO trigger RNA	polymer	120	38577.8	1	GenBank (2994212338)	Homo sapiens
7	J, K (N, M)	Zinc finger SWIM domain-containing protein 8	polymer	1866	200458.0	2	UniProt (A7E2V4)	Homo sapiens (human)
8	L, M (N, M)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A: 2 - 859 (UniProt: Q9UKV8)

PDB	External Database	Details
Met -10	-	initiating methionine
Gly -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag

H, I: 1 - 390 (UniProt: Q13618)

PDB	External Database	Details
Arg 342	Ile 342	engineered mutation
Asp 346	Leu 346	engineered mutation
Glu 391	-	expression tag
Asn 392	-	expression tag
Leu 393	-	expression tag
Tyr 394	-	expression tag
Phe 395	-	expression tag
Gln 396	-	expression tag

T: 2 - 116 (GenBank: 2994212338)

PDB	External Database	Details
G 1	-	expression tag
A 117	-	expression tag
A 118	-	expression tag
A 119	-	expression tag
A 120	-	expression tag

N, M: 1 - 1837 (UniProt: A7E2V4)

PDB	External Database	Details
Gly 1838	-	expression tag
Trp 1839	-	expression tag
Ser 1840	-	expression tag
His 1841	-	expression tag
Pro 1842	-	expression tag
Gln 1843	-	expression tag
Phe 1844	-	expression tag
Glu 1845	-	expression tag
Lys 1846	-	expression tag
Gly 1847	-	expression tag
Gly 1848	-	expression tag
Gly 1849	-	expression tag
Ser 1850	-	expression tag
Gly 1851	-	expression tag
Gly 1852	-	expression tag
Gly 1853	-	expression tag
Ser 1854	-	expression tag
Gly 1855	-	expression tag
Gly 1856	-	expression tag
Ser 1857	-	expression tag
Ala 1858	-	expression tag
Trp 1859	-	expression tag
Ser 1860	-	expression tag
His 1861	-	expression tag
Pro 1862	-	expression tag
Gln 1863	-	expression tag
Phe 1864	-	expression tag
Glu 1865	-	expression tag
Lys 1866	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	689824.8
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	689955.6

*Water molecules are not included.