Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

9RWZ

ZSWIM8-CUL3 complex bound to AGO2-miR-7-CYRANO

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000340	molecular_function	RNA 7-methylguanosine cap binding
A	0000932	cellular_component	P-body
A	0000993	molecular_function	RNA polymerase II complex binding
A	0001046	molecular_function	core promoter sequence-specific DNA binding
A	0003723	molecular_function	RNA binding
A	0003725	molecular_function	double-stranded RNA binding
A	0003727	molecular_function	single-stranded RNA binding
A	0003729	molecular_function	mRNA binding
A	0003743	molecular_function	translation initiation factor activity
A	0004521	molecular_function	RNA endonuclease activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006412	biological_process	translation
A	0006413	biological_process	translational initiation
A	0016020	cellular_component	membrane
A	0016442	cellular_component	RISC complex
A	0016891	molecular_function	RNA endonuclease activity producing 5'-phosphomonoesters, hydrolytic mechanism
A	0030425	cellular_component	dendrite
A	0031047	biological_process	regulatory ncRNA-mediated gene silencing
A	0031054	biological_process	pre-miRNA processing
A	0033962	biological_process	P-body assembly
A	0035194	biological_process	regulatory ncRNA-mediated post-transcriptional gene silencing
A	0035196	biological_process	miRNA processing
A	0035197	molecular_function	siRNA binding
A	0035198	molecular_function	miRNA binding
A	0035278	biological_process	miRNA-mediated gene silencing by inhibition of translation
A	0035279	biological_process	miRNA-mediated gene silencing by mRNA destabilization
A	0035925	molecular_function	mRNA 3'-UTR AU-rich region binding
A	0036464	cellular_component	cytoplasmic ribonucleoprotein granule
A	0042985	biological_process	negative regulation of amyloid precursor protein biosynthetic process
A	0045727	biological_process	positive regulation of translation
A	0045766	biological_process	positive regulation of angiogenesis
A	0045944	biological_process	positive regulation of transcription by RNA polymerase II
A	0045947	biological_process	negative regulation of translational initiation
A	0060213	biological_process	positive regulation of nuclear-transcribed mRNA poly(A) tail shortening
A	0070062	cellular_component	extracellular exosome
A	0070551	molecular_function	endoribonuclease activity, cleaving siRNA-paired mRNA
A	0070578	cellular_component	RISC-loading complex
A	0070922	biological_process	RISC complex assembly
A	0090128	biological_process	regulation of synapse maturation
A	0090624	molecular_function	endoribonuclease activity, cleaving miRNA-paired mRNA
A	0090625	biological_process	siRNA-mediated gene silencing by mRNA destabilization
A	0098794	cellular_component	postsynapse
A	0098808	molecular_function	mRNA cap binding
A	0098978	cellular_component	glutamatergic synapse
A	1900153	biological_process	positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
A	1901165	biological_process	positive regulation of trophoblast cell migration
A	1990904	cellular_component	ribonucleoprotein complex
B	0000122	biological_process	negative regulation of transcription by RNA polymerase II
B	0000151	cellular_component	ubiquitin ligase complex
B	0001222	molecular_function	transcription corepressor binding
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006367	biological_process	transcription initiation at RNA polymerase II promoter
B	0006368	biological_process	transcription elongation by RNA polymerase II
B	0016567	biological_process	protein ubiquitination
B	0030891	cellular_component	VCB complex
B	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
B	0031463	cellular_component	Cul3-RING ubiquitin ligase complex
B	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
B	0031625	molecular_function	ubiquitin protein ligase binding
B	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
B	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
B	0065003	biological_process	protein-containing complex assembly
B	0070449	cellular_component	elongin complex
B	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
B	0140958	biological_process	target-directed miRNA degradation
B	1990116	biological_process	ribosome-associated ubiquitin-dependent protein catabolic process
B	2000104	biological_process	negative regulation of DNA-templated DNA replication
C	0000122	biological_process	negative regulation of transcription by RNA polymerase II
C	0000151	cellular_component	ubiquitin ligase complex
C	0001222	molecular_function	transcription corepressor binding
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006367	biological_process	transcription initiation at RNA polymerase II promoter
C	0006368	biological_process	transcription elongation by RNA polymerase II
C	0016567	biological_process	protein ubiquitination
C	0030891	cellular_component	VCB complex
C	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
C	0031463	cellular_component	Cul3-RING ubiquitin ligase complex
C	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
C	0031625	molecular_function	ubiquitin protein ligase binding
C	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
C	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
C	0065003	biological_process	protein-containing complex assembly
C	0070449	cellular_component	elongin complex
C	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
C	0140958	biological_process	target-directed miRNA degradation
C	1990116	biological_process	ribosome-associated ubiquitin-dependent protein catabolic process
C	2000104	biological_process	negative regulation of DNA-templated DNA replication
D	0000122	biological_process	negative regulation of transcription by RNA polymerase II
D	0000151	cellular_component	ubiquitin ligase complex
D	0001222	molecular_function	transcription corepressor binding
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006357	biological_process	regulation of transcription by RNA polymerase II
D	0006367	biological_process	transcription initiation at RNA polymerase II promoter
D	0006511	biological_process	ubiquitin-dependent protein catabolic process
D	0016567	biological_process	protein ubiquitination
D	0030674	molecular_function	protein-macromolecule adaptor activity
D	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
D	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
D	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
D	0070449	cellular_component	elongin complex
D	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
D	0140958	biological_process	target-directed miRNA degradation
D	1990116	biological_process	ribosome-associated ubiquitin-dependent protein catabolic process
D	2000104	biological_process	negative regulation of DNA-templated DNA replication
E	0000122	biological_process	negative regulation of transcription by RNA polymerase II
E	0000151	cellular_component	ubiquitin ligase complex
E	0001222	molecular_function	transcription corepressor binding
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006357	biological_process	regulation of transcription by RNA polymerase II
E	0006367	biological_process	transcription initiation at RNA polymerase II promoter
E	0006511	biological_process	ubiquitin-dependent protein catabolic process
E	0016567	biological_process	protein ubiquitination
E	0030674	molecular_function	protein-macromolecule adaptor activity
E	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
E	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
E	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
E	0070449	cellular_component	elongin complex
E	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
E	0140958	biological_process	target-directed miRNA degradation
E	1990116	biological_process	ribosome-associated ubiquitin-dependent protein catabolic process
E	2000104	biological_process	negative regulation of DNA-templated DNA replication
M	0005829	cellular_component	cytosol
M	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
M	0016567	biological_process	protein ubiquitination
M	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
M	0031463	cellular_component	Cul3-RING ubiquitin ligase complex
M	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
M	0140958	biological_process	target-directed miRNA degradation
M	1990756	molecular_function	ubiquitin-like ligase-substrate adaptor activity
M	2000627	biological_process	positive regulation of miRNA catabolic process
N	0005829	cellular_component	cytosol
N	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
N	0016567	biological_process	protein ubiquitination
N	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
N	0031463	cellular_component	Cul3-RING ubiquitin ligase complex
N	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
N	0140958	biological_process	target-directed miRNA degradation
N	1990756	molecular_function	ubiquitin-like ligase-substrate adaptor activity
N	2000627	biological_process	positive regulation of miRNA catabolic process

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	119
Details	Domain: {"description":"PAZ","evidences":[{"source":"PROSITE-ProRule","id":"PRU00142","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	301
Details	Domain: {"description":"Piwi","evidences":[{"source":"HAMAP-Rule","id":"MF_03031","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	78
Details	Region: {"description":"Interaction with guide RNA"}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	13
Details	Region: {"description":"Interaction with GW182 family members","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	3
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"23603119","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	1
Details	Modified residue: {"description":"4-hydroxyproline","evidences":[{"source":"HAMAP-Rule","id":"MF_03031","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"18690212","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	130
Details	Domain: {"description":"Ubiquitin-like","evidences":[{"source":"PROSITE-ProRule","id":"PRU00214","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	2
Details	Modified residue: {"description":"N-acetylmethionine","evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"MAR-2009","submissionDatabase":"UniProtKB","authors":["Bienvenut W.V.","Waridel P.","Quadroni M."]}},{"source":"PubMed","id":"19413330","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"22814378","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	2
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"UniProtKB","id":"P62869","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI11
Number of Residues	72
Details	Zinc finger: {"description":"SWIM-type","evidences":[{"source":"PROSITE-ProRule","id":"PRU00325","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI12
Number of Residues	40
Details	Region: {"description":"Disordered","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

site_id	SWS_FT_FI13
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI14
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"20068231","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)