9O6B

X-Ray structure of F240L mutant HOXB13 in complex with DNA (CAA)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (J, A)	Homeobox protein Hox-B13	polymer	101	11616.5	2	UniProt (Q92826) Pfam (PF00046)	Homo sapiens (human)
2	B, E (K, B)	DNA-TTGTGTTTTATTGGGTCC	polymer	18	5534.6	2		synthetic construct
3	C, F (L, C)	DNA-GGACCCAATAAAACACAA	polymer	18	5495.6	2		synthetic construct
4	G, H, I, J, K... (J, K, L, A, B...)	water	water		18.0	131	Chemie (HOH)

Sequence modifications

J, A: 217 - 284 (UniProt: Q92826)

PDB	External Database	Details
Met 184	-	initiating methionine
Gly 185	-	expression tag
Ser 186	-	expression tag
Ser 187	-	expression tag
His 188	-	expression tag
His 189	-	expression tag
His 190	-	expression tag
His 191	-	expression tag
His 192	-	expression tag
His 193	-	expression tag
Ser 194	-	expression tag
Ser 195	-	expression tag
Gly 196	-	expression tag
Leu 197	-	expression tag
Val 198	-	expression tag
Pro 199	-	expression tag
Arg 200	-	expression tag
Gly 201	-	expression tag
Ser 202	-	expression tag
His 203	-	expression tag
Met 204	-	expression tag
Ala 205	-	expression tag
Ser 206	-	expression tag
Met 207	-	expression tag
Thr 208	-	expression tag
Gly 209	-	expression tag
Gly 210	-	expression tag
Gln 211	-	expression tag
Gln 212	-	expression tag
Met 213	-	expression tag
Gly 214	-	expression tag
Arg 215	-	expression tag
Met 216	-	expression tag
Leu 240	Phe 240	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	45293.5
All*	Total formula weight	45293.5

*Water molecules are not included.