9O6B

X-Ray structure of F240L mutant HOXB13 in complex with DNA (CAA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-20
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97946
Spacegroup name	C 1 2 1
Unit cell lengths	113.220, 57.820, 75.490
Unit cell angles	90.00, 119.00, 90.00

Refinement procedure

Resolution	33.010 - 1.950
R-factor	0.2284
Rwork	0.226
R-free	0.27340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.918
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.830	2.000
High resolution limit [Å]	1.950	1.950
Number of reflections	30715	2269
<I/σ(I)>	15.5	1.3
Completeness [%]	98.3	98.4
Redundancy	7	7
CC(1/2)	1.000	0.712

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	295	24% PEG3350, 150mM KCl, 100mM MgCl2, Tris pH8.0