9O04

CryoEM structure of the FBXO42-CCDC6-PP2Ac degradasome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Coiled-coil domain-containing protein 6	polymer	490	55323.0	2	UniProt (Q16204) Pfam (PF09755)	Homo sapiens (human)	Papillary thyroid carcinoma-encoded protein,Protein H4
2	C, G (C, G)	F-box only protein 42	polymer	691	75126.9	2	UniProt (Q6P3S6) Pfam (PF12937) Pfam (PF13415)	Homo sapiens (human)	Just one F-box and Kelch domain-containing protein
3	D, E, I, J, K... (D, E, I, J, K...)	Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform	polymer	336	38858.5	8	UniProt (P67775) Pfam (PF00149)	Homo sapiens (human)	PP2A-alpha,Replication protein C,RP-C
4	F, H (F, H)	SKP1	polymer	151	16997.3	2		Homo sapiens
5	AA, BA, CA, DA, O... (L, M, N, B, D...)	MANGANESE (II) ION	non-polymer		54.9	16	Chemie (MN)

Sequence modifications

A, B: 1 - 474 (UniProt: Q16204)

PDB	External Database	Details
Met -15	-	initiating methionine
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Gly -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

C, G: 44 - 717 (UniProt: Q6P3S6)

PDB	External Database	Details
Met 27	-	initiating methionine
Asp 28	-	expression tag
Tyr 29	-	expression tag
Lys 30	-	expression tag
Asp 31	-	expression tag
Asp 32	-	expression tag
Asp 33	-	expression tag
Asp 34	-	expression tag
Lys 35	-	expression tag
Gly 36	-	expression tag
Glu 37	-	expression tag
Asn 38	-	expression tag
Leu 39	-	expression tag
Tyr 40	-	expression tag
Phe 41	-	expression tag
Gln 42	-	expression tag
Gly 43	-	expression tag
Ser 44	Asn 44	conflict

D, E, I, J, K, L, M, N: 1 - 309 (UniProt: P67775)

PDB	External Database	Details
Met -26	-	initiating methionine
Asp -25	-	expression tag
Tyr -24	-	expression tag
Lys -23	-	expression tag
Asp -22	-	expression tag
Asp -21	-	expression tag
Asp -20	-	expression tag
Asp -19	-	expression tag
Lys -18	-	expression tag
Gly -17	-	expression tag
Glu -16	-	expression tag
Asn -15	-	expression tag
Leu -14	-	expression tag
Tyr -13	-	expression tag
Phe -12	-	expression tag
Gln -11	-	expression tag
Gly -10	-	expression tag
Ser -9	-	expression tag
Tyr -8	-	expression tag
Pro -7	-	expression tag
Tyr -6	-	expression tag
Asp -5	-	expression tag
Val -4	-	expression tag
Pro -3	-	expression tag
Asp -2	-	expression tag
Tyr -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	605762.2
Non-Polymers*	Number of molecules	16
	Total formula weight	879.0
All*	Total formula weight	606641.2

*Water molecules are not included.