9MPP

The cryo-EM structure of nucleosome-bound DNA methyltransferases DNMT3A2 and DNMT3L

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Histone H3.2	polymer	135	15303.9	2	UniProt (P84233)	Xenopus laevis (African clawed frog)	Histone H3
2	B, F (B, F)	Histone H4	polymer	103	11394.4	2	UniProt (P62799)	Xenopus laevis (African clawed frog)
3	C, G (C, G)	Histone H2A type 1	polymer	129	13978.2	2	UniProt (P06897)	Xenopus laevis (African clawed frog)
4	D, H (D, H)	Histone H2B 1.1	polymer	123	13655.9	2	UniProt (P02281)	Xenopus laevis (African clawed frog)	H2B1.1
5	I (I)	DNA (167-MER)	polymer	167	51327.7	1		synthetic construct
6	J (J)	DNA (167-MER)	polymer	167	51786.0	1		synthetic construct
7	K, M (M, K)	DNA (cytosine-5)-methyltransferase 3A	polymer	689	77914.7	2	UniProt (Q9Y6K1)	Homo sapiens (human)	Dnmt3a,DNA methyltransferase HsaIIIA,M.HsaIIIA
8	L, N (N, L)	DNA (cytosine-5)-methyltransferase 3-like	polymer	386	43534.6	2	UniProt (Q9UJW3)	Homo sapiens (human)
9	O, P, Q, S, T... (M, N)	ZINC ION	non-polymer		65.4	6	Chemie (ZN)
10	R, V (M, K)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	2	Chemie (SAH)

Sequence modifications

A, E: 1 - 135 (UniProt: P84233)

PDB	External Database	Details
Ala 102	Gly 103	variant

C, G: 1 - 129 (UniProt: P06897)

PDB	External Database	Details
Arg 99	Gly 100	engineered mutation
Ser 123	Ala 124	engineered mutation

D, H: 1 - 122 (UniProt: P02281)

PDB	External Database	Details
Met 0	-	initiating methionine
Thr 29	Ser 33	engineered mutation

N, L: 1 - 386 (UniProt: Q9UJW3)

PDB	External Database	Details
Thr 5	Pro 5	conflict
Gly 278	Arg 278	variant

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	454677.4
Non-Polymers*	Number of molecules	8
	Total formula weight	1161.3
All*	Total formula weight	455838.7

*Water molecules are not included.