9MK3
Engineered AsCas12a (M537R, F870L)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E (A, E) | CRISPR-associated endonuclease Cas12a | polymer | 1307 | 151410.9 | 2 | UniProt (U2UMQ6) | Acidaminococcus | AsCpf1,CRISPR-associated endonuclease Cpf1 |
| 2 | B, F (B, F) | gRNA (41-MER) | polymer | 44 | 14062.3 | 2 | Acidaminococcus | ||
| 3 | C, G (C, G) | TS DNA (31-MER) | polymer | 34 | 10452.7 | 2 | Acidaminococcus | ||
| 4 | D, H (D, H) | NTS DNA (5'-D(*CP*AP*GP*TP*CP*CP*TP*TP*TP*T)-3') | polymer | 10 | 2986.0 | 2 | Acidaminococcus | ||
| 5 | I, R (A, E) | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
| 6 | J, S (A, E) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 7 | K, L, M, N, O... (A, E, G) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 11 | Chemie (EDO) | |||
| 8 | AA, BA, CA, X, Y... (E, F, G, A, B...) | water | water | 18.0 | 137 | Chemie (HOH) |
Sequence modifications
A, E: 1 - 1307 (UniProt: U2UMQ6)
| PDB | External Database | Details |
|---|---|---|
| Arg 537 | Met 537 | engineered mutation |
| Leu 870 | Phe 870 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 357823.9 | |
| Non-Polymers* | Number of molecules | 15 |
| Total formula weight | 799.6 | |
| All* | Total formula weight | 358623.5 |
