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SummaryStructural detailsExperimental detailsFunctional detailsSequence NeighborHistoryDownloads

9MK3

Engineered AsCas12a (M537R, F870L)

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues16
DetailsDNA binding: {"description":"PAM-binding on target DNA","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27444870","evidenceCode":"ECO:0000269"}]}
ChainResidueDetails
site_idSWS_FT_FI2
Number of Residues44
DetailsDNA binding: {"description":"Target DNA","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27444870","evidenceCode":"ECO:0000269"}]}
ChainResidueDetails
site_idSWS_FT_FI3
Number of Residues68
DetailsRegion: {"description":"WED-I (OBD-I)","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"27444870","evidenceCode":"ECO:0000303"}]}
ChainResidueDetails
site_idSWS_FT_FI4
Number of Residues568
DetailsRegion: {"description":"REC1 (helical-I)","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"27444870","evidenceCode":"ECO:0000303"}]}
ChainResidueDetails
site_idSWS_FT_FI5
Number of Residues410
DetailsRegion: {"description":"WED-II (helical-II)","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"27444870","evidenceCode":"ECO:0000303"}]}
ChainResidueDetails
site_idSWS_FT_FI6
Number of Residues142
DetailsRegion: {"description":"WED-II (OBD-I)","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"27444870","evidenceCode":"ECO:0000303"}]}
ChainResidueDetails
site_idSWS_FT_FI7
Number of Residues238
DetailsRegion: {"description":"PI (LHD)","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"27444870","evidenceCode":"ECO:0000303"}]}
ChainResidueDetails
site_idSWS_FT_FI8
Number of Residues330
DetailsRegion: {"description":"WED-III (OBD-III)","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"27444870","evidenceCode":"ECO:0000303"}]}
ChainResidueDetails
site_idSWS_FT_FI9
Number of Residues110
DetailsRegion: {"description":"RuvC-I","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"27444870","evidenceCode":"ECO:0000303"}]}
ChainResidueDetails
site_idSWS_FT_FI10
Number of Residues32
DetailsRegion: {"description":"Bridge helix","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"27444870","evidenceCode":"ECO:0000303"}]}
ChainResidueDetails
site_idSWS_FT_FI11
Number of Residues88
DetailsRegion: {"description":"RuvC-III","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"27444870","evidenceCode":"ECO:0000303"}]}
ChainResidueDetails
site_idSWS_FT_FI12
Number of Residues64
DetailsCoiled coil: {"evidences":[{"evidenceCode":"ECO:0000255"}]}
ChainResidueDetails
site_idSWS_FT_FI13
Number of Residues6
DetailsActive site: {"description":"For pre-crRNA processing","evidences":[{"source":"UniProtKB","id":"A0Q7Q2","evidenceCode":"ECO:0000250"}]}
ChainResidueDetails
site_idSWS_FT_FI14
Number of Residues6
DetailsActive site: {"description":"For DNase activity of RuvC domain","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000269"}]}
ChainResidueDetails
site_idSWS_FT_FI15
Number of Residues2
DetailsActive site: {"description":"For DNase activity of nuclease domain","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000269"}]}
ChainResidueDetails
site_idSWS_FT_FI16
Number of Residues34
DetailsBinding site: {"evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27444870","evidenceCode":"ECO:0000269"}]}
ChainResidueDetails
site_idSWS_FT_FI17
Number of Residues4
DetailsBinding site: {"evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000269"}]}
ChainResidueDetails
site_idSWS_FT_FI18
Number of Residues6
DetailsSite: {"description":"Binds crRNA","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27444870","evidenceCode":"ECO:0000269"}]}
ChainResidueDetails
site_idSWS_FT_FI19
Number of Residues4
DetailsSite: {"description":"Binds PAM on target DNA","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27444870","evidenceCode":"ECO:0000269"}]}
ChainResidueDetails
site_idSWS_FT_FI20
Number of Residues2
DetailsSite: {"description":"Binds crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27444870","evidenceCode":"ECO:0000269"}]}
ChainResidueDetails
site_idSWS_FT_FI21
Number of Residues2
DetailsSite: {"description":"Binds sequence-specific recognition of both target and non-target strand bases in PAM","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27444870","evidenceCode":"ECO:0000269"}]}
ChainResidueDetails
site_idSWS_FT_FI22
Number of Residues2
DetailsSite: {"description":"Binds target DNA","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000269"}]}
ChainResidueDetails
site_idSWS_FT_FI23
Number of Residues195
DetailsRegion: {"description":"Nuclease domain","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"27444870","evidenceCode":"ECO:0000303"}]}
ChainResidueDetails

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PDB entries from 2026-03-18

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