9MB6

Cryo-EM structure of wild-type SaCas9-guide RNA-mismatched target DNA complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (C)	target DNA strand	polymer	43	13305.0	1		Staphylococcus aureus
2	B (A)	Ubiquitin-like protein SMT3,CRISPR-associated endonuclease Cas9	polymer	1170	137650.6	1	UniProt (Q12306) UniProt (J7RUA5) Pfam (PF00240)	Staphylococcus aureus	SaCas9
3	C (B)	sgRNA	polymer	99	31892.9	1		Staphylococcus aureus
4	D (D)	non-target DNA strand	polymer	43	13434.1	1		Staphylococcus aureus

Sequence modifications

A: -104 - -9 (UniProt: Q12306)

PDB	External Database	Details
Met -116	-	initiating methionine
Gly -115	-	expression tag
His -114	-	expression tag
His -113	-	expression tag
His -112	-	expression tag
His -111	-	expression tag
His -110	-	expression tag
His -109	-	expression tag
Gly -108	-	expression tag
Ser -107	-	expression tag
Leu -106	-	expression tag
Gln -105	-	expression tag
Ala -30	Thr 77	conflict
Glu -8	-	linker
Asn -7	-	linker
Leu -6	-	linker
Tyr -5	-	linker
Phe -4	-	linker
Gln -3	-	linker
Gly -2	-	linker
Ser -1	-	linker
His 0	-	linker

A: 1 - 1053 (UniProt: J7RUA5)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	196282.5
All*	Total formula weight	196282.5

*Water molecules are not included.