9MB6
Cryo-EM structure of wild-type SaCas9-guide RNA-mismatched target DNA complex
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000794 | cellular_component | condensed nuclear chromosome |
| A | 0004519 | molecular_function | endonuclease activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005940 | cellular_component | septin ring |
| A | 0016925 | biological_process | protein sumoylation |
| A | 0031386 | molecular_function | protein tag activity |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0043571 | biological_process | maintenance of CRISPR repeat elements |
| A | 0044389 | molecular_function | ubiquitin-like protein ligase binding |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0051607 | biological_process | defense response to virus |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 166 |
| Details | Domain: {"description":"HNH Cas9-type","evidences":[{"source":"HAMAP-Rule","id":"MF_01480","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 40 |
| Details | Region: {"description":"RuvC-I","evidences":[{"source":"PubMed","id":"26317473","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 46 |
| Details | Region: {"description":"RuvC-II","evidences":[{"source":"PubMed","id":"26317473","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 15 |
| Details | Region: {"description":"PAM substrate-binding","evidences":[{"source":"PubMed","id":"26317473","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 143 |
| Details | Region: {"description":"PAM-interacting domain (PI)","evidences":[{"source":"PubMed","id":"26317473","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | Active site: {"description":"For RuvC-like nuclease domain","evidences":[{"source":"PubMed","id":"26317473","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | Active site: {"description":"Proton acceptor for HNH nuclease domain","evidences":[{"source":"PubMed","id":"26317473","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 4 |
| Details | Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01480","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 1 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"26317473","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
