9IP2

Cryo-EM structure of the RNA-dependent RNA polymerase complex from Marburg virus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	RNA-directed RNA polymerase L,Maltose/maltodextrin-binding periplasmic protein	polymer	2757	313963.7	1	UniProt (P31352) UniProt (P0AEX9) Pfam (PF00946) Pfam (PF14318)	Marburg virus - Musoke Kenya 1980	Protein L,Large structural protein,Replicase,Transcriptase,MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP
2	B, C, D, E (B, C, D, E)	Maltose/maltodextrin-binding periplasmic protein,Polymerase cofactor VP35	polymer	727	79881.0	4	UniProt (P0AEX9) UniProt (P35259) Pfam (PF02097)	Escherichia coli K-12	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP,Marburg VP35,mVP35
3	F (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

A: 1 - 2331 (UniProt: P31352)

PDB	External Database	Details
Ala 489	Leu 489	conflict
Gly 979	Arg 979	conflict
Thr 1428	Ser 1428	conflict
Ser 2332	-	linker
Arg 2333	-	linker
Glu 2334	-	linker
Asn 2335	-	linker
Leu 2336	-	linker
Tyr 2337	-	linker
Phe 2338	-	linker
Gln 2339	-	linker
Gly 2340	-	linker
Ser 2341	-	linker
Gly 2342	-	linker
Trp 2343	-	linker
Ser 2344	-	linker
His 2345	-	linker
Pro 2346	-	linker
Gln 2347	-	linker
Phe 2348	-	linker
Glu 2349	-	linker
Lys 2350	-	linker
Gly 2351	-	linker
Gly 2352	-	linker
Gly 2353	-	linker
Ser 2354	-	linker
Gly 2355	-	linker
Gly 2356	-	linker
Gly 2357	-	linker
Ser 2358	-	linker
Gly 2359	-	linker
Gly 2360	-	linker
Ser 2361	-	linker
Ala 2362	-	linker
Trp 2363	-	linker
Ser 2364	-	linker
His 2365	-	linker
Pro 2366	-	linker
Gln 2367	-	linker
Phe 2368	-	linker
Glu 2369	-	linker
Lys 2370	-	linker
Gly 2371	-	linker
Ser 2372	-	linker
Ala 2373	-	linker
Ser 2374	-	linker
His 2375	-	linker
His 2376	-	linker
His 2377	-	linker
His 2378	-	linker
His 2379	-	linker
His 2380	-	linker
Gly 2381	-	linker
Thr 2382	-	linker
Lys 2383	-	linker
Thr 2384	-	linker

A: 2385 - 2748 (UniProt: P0AEX9)

PDB	External Database	Details
Gly 2749	-	expression tag
Asp 2750	-	expression tag
Tyr 2751	-	expression tag
Lys 2752	-	expression tag
Asp 2753	-	expression tag
Asp 2754	-	expression tag
Asp 2755	-	expression tag
Asp 2756	-	expression tag
Lys 2757	-	expression tag

B, C, D, E: -383 - -20 (UniProt: P0AEX9)

PDB	External Database	Details
Met -397	-	initiating methionine
Gly -396	-	expression tag
Ser -395	-	expression tag
Ser -394	-	expression tag
His -393	-	expression tag
His -392	-	expression tag
His -391	-	expression tag
His -390	-	expression tag
His -389	-	expression tag
His -388	-	expression tag
Gly -387	-	expression tag
Thr -386	-	expression tag
Lys -385	-	expression tag
Thr -384	-	expression tag
Gly -19	-	linker
Thr -18	-	linker
Asp -17	-	linker
Tyr -16	-	linker
Asp -15	-	linker
Ile -14	-	linker
Pro -13	-	linker
Thr -12	-	linker
Thr -11	-	linker
Leu -10	-	linker
Glu -9	-	linker
Val -8	-	linker
Leu -7	-	linker
Phe -6	-	linker
Gln -5	-	linker
Gly -4	-	linker
Pro -3	-	linker
Leu -2	-	linker
Gly -1	-	linker
Ser 0	-	linker

B, C, D, E: 1 - 329 (UniProt: P35259)

PDB	External Database	Details
Cys 296	Ser 296	conflict

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	633487.5
Non-Polymers*	Number of molecules	1
	Total formula weight	65.4
All*	Total formula weight	633552.9

*Water molecules are not included.