9IAZ

CryoEM structure of the Themis:Grb2 complex with bound ProMacrobody 256, local refinement

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Growth factor receptor-bound protein 2	polymer	224	26198.4	1	UniProt (P62993) Pfam (PF00018) Pfam (PF00017)	Homo sapiens (human)	Adapter protein GRB2,Protein Ash,SH2/SH3 adapter GRB2
2	B (B)	Protein THEMIS	polymer	641	73550.3	1	UniProt (Q8N1K5) Pfam (PF12736)	Homo sapiens (human)	Thymocyte-expressed molecule involved in selection
3	C (C)	ProMacrobody 256	polymer	478	52516.0	1	UniProt (P0AEY0) Pfam (PF01547)	synthetic construct	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP

Sequence modifications

A: 1 - 217 (UniProt: P62993)

PDB	External Database	Details
Lys 218	-	expression tag
His 219	-	expression tag
His 220	-	expression tag
His 221	-	expression tag
His 222	-	expression tag
His 223	-	expression tag
His 224	-	expression tag

C: 1 - 116 (PDB: 9IAZ)

PDB	External Database	Details
Pro 117	-	linker
Pro 118	-	linker

C: 119 - 478 (UniProt: P0AEY0)

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	152264.7
All*	Total formula weight	152264.7

*Water molecules are not included.