9IAZ
CryoEM structure of the Themis:Grb2 complex with bound ProMacrobody 256, local refinement
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM GP |
EM Data Collection
| Microscope | JEOL CRYO ARM 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.2 |
| Software | cryoSPARC,PHENIX,crYOLO,SerialEM,UCSF ChimeraX,NAMD |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
