9HJC

Cryo-EM structure of thiamine-bound human TPK1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Thiamine pyrophosphokinase 1	polymer	277	31091.3	2	UniProt (Q9H3S4) Pfam (PF04263) Pfam (PF04265)	Homo sapiens (human)	hTPK1,Placental protein 20,PP20,Thiamin pyrophosphokinase 1
2	C, E (A, B)	3-(4-AMINO-2-METHYL-PYRIMIDIN-5-YLMETHYL)-5-(2-HYDROXY-ETHYL)-4-METHYL-THIAZOL-3-IUM	non-polymer		265.4	2	Chemie (VIB)
3	D, F (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

A, B: 1 - 243 (UniProt: Q9H3S4)

PDB	External Database	Details
Leu 244	-	expression tag
Glu 245	-	expression tag
Val 246	-	expression tag
Leu 247	-	expression tag
Phe 248	-	expression tag
Gln 249	-	expression tag
Gly 250	-	expression tag
Pro 251	-	expression tag
Ala 252	-	expression tag
Gly 253	-	expression tag
Arg 254	-	expression tag
Ala 255	-	expression tag
Gly 256	-	expression tag
Glu 257	-	expression tag
Gln 258	-	expression tag
Lys 259	-	expression tag
Leu 260	-	expression tag
Ile 261	-	expression tag
Ser 262	-	expression tag
Glu 263	-	expression tag
Glu 264	-	expression tag
Asp 265	-	expression tag
Leu 266	-	expression tag
Asn 267	-	expression tag
Ser 268	-	expression tag
Ala 269	-	expression tag
Val 270	-	expression tag
Asp 271	-	expression tag
His 272	-	expression tag
His 273	-	expression tag
His 274	-	expression tag
His 275	-	expression tag
His 276	-	expression tag
His 277	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	62182.7
Non-Polymers*	Number of molecules	4
	Total formula weight	579.3
All*	Total formula weight	62762.0

*Water molecules are not included.