9GY0
Cryo_EM structure of human FAN1 R507H mutant in complex with 5' flap DNA substrate and PCNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Fanconi-associated nuclease 1 | polymer | 1021 | 114705.2 | 1 | UniProt (Q9Y2M0) Pfam (PF21315) Pfam (PF21169) Pfam (PF21170) Pfam (PF08774) | Homo sapiens (human) | FANCD2/FANCI-associated nuclease 1,hFAN1,Myotubularin-related protein 15 |
| 2 | B (B) | DNA (Continuous) | polymer | 40 | 12154.7 | 1 | Homo sapiens | ||
| 3 | C (C) | DNA (Pre-Nick) | polymer | 28 | 8618.5 | 1 | Homo sapiens | ||
| 4 | D (D) | DNA (Post-Nick) | polymer | 21 | 6554.2 | 1 | Homo sapiens | ||
| 5 | E, F, G (E, F, G) | Proliferating cell nuclear antigen | polymer | 263 | 28949.9 | 3 | UniProt (P12004) Pfam (PF00705) Pfam (PF02747) | Homo sapiens (human) | PCNA,Cyclin |
| 6 | H (A) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) |
Sequence modifications
A: 1 - 1017 (UniProt: Q9Y2M0)
E, F, G: 1 - 261 (UniProt: P12004)
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Pro -2 | - | expression tag |
| Met -1 | - | expression tag |
| Gly 0 | - | expression tag |
| His 507 | Arg 507 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Pro 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 7 |
| Total formula weight | 228882.4 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 40.1 | |
| All* | Total formula weight | 228922.5 |
