9GY0

Cryo_EM structure of human FAN1 R507H mutant in complex with 5' flap DNA substrate and PCNA

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0000724	biological_process	double-strand break repair via homologous recombination
A	0003676	molecular_function	nucleic acid binding
A	0003677	molecular_function	DNA binding
A	0004518	molecular_function	nuclease activity
A	0004519	molecular_function	endonuclease activity
A	0004527	molecular_function	exonuclease activity
A	0004528	molecular_function	phosphodiesterase I activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0006281	biological_process	DNA repair
A	0006289	biological_process	nucleotide-excision repair
A	0006974	biological_process	DNA damage response
A	0008270	molecular_function	zinc ion binding
A	0008409	molecular_function	5'-3' exonuclease activity
A	0016787	molecular_function	hydrolase activity
A	0016788	molecular_function	hydrolase activity, acting on ester bonds
A	0017108	molecular_function	5'-flap endonuclease activity
A	0036297	biological_process	interstrand cross-link repair
A	0046872	molecular_function	metal ion binding
A	0070336	molecular_function	flap-structured DNA binding
A	0140036	molecular_function	ubiquitin-modified protein reader activity
E	0000307	cellular_component	cyclin-dependent protein kinase holoenzyme complex
E	0000701	molecular_function	purine-specific mismatch base pair DNA N-glycosylase activity
E	0000781	cellular_component	chromosome, telomeric region
E	0000785	cellular_component	chromatin
E	0003677	molecular_function	DNA binding
E	0003682	molecular_function	chromatin binding
E	0003684	molecular_function	damaged DNA binding
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005652	cellular_component	nuclear lamina
E	0005654	cellular_component	nucleoplasm
E	0005657	cellular_component	replication fork
E	0005813	cellular_component	centrosome
E	0006260	biological_process	DNA replication
E	0006272	biological_process	leading strand elongation
E	0006275	biological_process	regulation of DNA replication
E	0006281	biological_process	DNA repair
E	0006298	biological_process	mismatch repair
E	0006325	biological_process	chromatin organization
E	0006974	biological_process	DNA damage response
E	0006979	biological_process	response to oxidative stress
E	0007507	biological_process	heart development
E	0019899	molecular_function	enzyme binding
E	0019985	biological_process	translesion synthesis
E	0030331	molecular_function	nuclear estrogen receptor binding
E	0030337	molecular_function	DNA polymerase processivity factor activity
E	0030855	biological_process	epithelial cell differentiation
E	0030894	cellular_component	replisome
E	0030971	molecular_function	receptor tyrosine kinase binding
E	0031297	biological_process	replication fork processing
E	0032139	molecular_function	dinucleotide insertion or deletion binding
E	0032355	biological_process	response to estradiol
E	0032405	molecular_function	MutLalpha complex binding
E	0033993	biological_process	response to lipid
E	0034644	biological_process	cellular response to UV
E	0035035	molecular_function	histone acetyltransferase binding
E	0042802	molecular_function	identical protein binding
E	0043596	cellular_component	nuclear replication fork
E	0043626	cellular_component	PCNA complex
E	0044849	biological_process	estrous cycle
E	0044877	molecular_function	protein-containing complex binding
E	0045739	biological_process	positive regulation of DNA repair
E	0045740	biological_process	positive regulation of DNA replication
E	0046686	biological_process	response to cadmium ion
E	0070062	cellular_component	extracellular exosome
E	0070182	molecular_function	DNA polymerase binding
E	0070301	biological_process	cellular response to hydrogen peroxide
E	0070557	cellular_component	PCNA-p21 complex
E	0071548	biological_process	response to dexamethasone
E	0097421	biological_process	liver regeneration
E	0140861	biological_process	DNA repair-dependent chromatin remodeling
E	1902065	biological_process	response to L-glutamate
E	1902969	biological_process	mitotic DNA replication
E	1902990	biological_process	mitotic telomere maintenance via semi-conservative replication
F	0000307	cellular_component	cyclin-dependent protein kinase holoenzyme complex
F	0000701	molecular_function	purine-specific mismatch base pair DNA N-glycosylase activity
F	0000781	cellular_component	chromosome, telomeric region
F	0000785	cellular_component	chromatin
F	0003677	molecular_function	DNA binding
F	0003682	molecular_function	chromatin binding
F	0003684	molecular_function	damaged DNA binding
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005652	cellular_component	nuclear lamina
F	0005654	cellular_component	nucleoplasm
F	0005657	cellular_component	replication fork
F	0005813	cellular_component	centrosome
F	0006260	biological_process	DNA replication
F	0006272	biological_process	leading strand elongation
F	0006275	biological_process	regulation of DNA replication
F	0006281	biological_process	DNA repair
F	0006298	biological_process	mismatch repair
F	0006325	biological_process	chromatin organization
F	0006974	biological_process	DNA damage response
F	0006979	biological_process	response to oxidative stress
F	0007507	biological_process	heart development
F	0019899	molecular_function	enzyme binding
F	0019985	biological_process	translesion synthesis
F	0030331	molecular_function	nuclear estrogen receptor binding
F	0030337	molecular_function	DNA polymerase processivity factor activity
F	0030855	biological_process	epithelial cell differentiation
F	0030894	cellular_component	replisome
F	0030971	molecular_function	receptor tyrosine kinase binding
F	0031297	biological_process	replication fork processing
F	0032139	molecular_function	dinucleotide insertion or deletion binding
F	0032355	biological_process	response to estradiol
F	0032405	molecular_function	MutLalpha complex binding
F	0033993	biological_process	response to lipid
F	0034644	biological_process	cellular response to UV
F	0035035	molecular_function	histone acetyltransferase binding
F	0042802	molecular_function	identical protein binding
F	0043596	cellular_component	nuclear replication fork
F	0043626	cellular_component	PCNA complex
F	0044849	biological_process	estrous cycle
F	0044877	molecular_function	protein-containing complex binding
F	0045739	biological_process	positive regulation of DNA repair
F	0045740	biological_process	positive regulation of DNA replication
F	0046686	biological_process	response to cadmium ion
F	0070062	cellular_component	extracellular exosome
F	0070182	molecular_function	DNA polymerase binding
F	0070301	biological_process	cellular response to hydrogen peroxide
F	0070557	cellular_component	PCNA-p21 complex
F	0071548	biological_process	response to dexamethasone
F	0097421	biological_process	liver regeneration
F	0140861	biological_process	DNA repair-dependent chromatin remodeling
F	1902065	biological_process	response to L-glutamate
F	1902969	biological_process	mitotic DNA replication
F	1902990	biological_process	mitotic telomere maintenance via semi-conservative replication
G	0000307	cellular_component	cyclin-dependent protein kinase holoenzyme complex
G	0000701	molecular_function	purine-specific mismatch base pair DNA N-glycosylase activity
G	0000781	cellular_component	chromosome, telomeric region
G	0000785	cellular_component	chromatin
G	0003677	molecular_function	DNA binding
G	0003682	molecular_function	chromatin binding
G	0003684	molecular_function	damaged DNA binding
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005652	cellular_component	nuclear lamina
G	0005654	cellular_component	nucleoplasm
G	0005657	cellular_component	replication fork
G	0005813	cellular_component	centrosome
G	0006260	biological_process	DNA replication
G	0006272	biological_process	leading strand elongation
G	0006275	biological_process	regulation of DNA replication
G	0006281	biological_process	DNA repair
G	0006298	biological_process	mismatch repair
G	0006325	biological_process	chromatin organization
G	0006974	biological_process	DNA damage response
G	0006979	biological_process	response to oxidative stress
G	0007507	biological_process	heart development
G	0019899	molecular_function	enzyme binding
G	0019985	biological_process	translesion synthesis
G	0030331	molecular_function	nuclear estrogen receptor binding
G	0030337	molecular_function	DNA polymerase processivity factor activity
G	0030855	biological_process	epithelial cell differentiation
G	0030894	cellular_component	replisome
G	0030971	molecular_function	receptor tyrosine kinase binding
G	0031297	biological_process	replication fork processing
G	0032139	molecular_function	dinucleotide insertion or deletion binding
G	0032355	biological_process	response to estradiol
G	0032405	molecular_function	MutLalpha complex binding
G	0033993	biological_process	response to lipid
G	0034644	biological_process	cellular response to UV
G	0035035	molecular_function	histone acetyltransferase binding
G	0042802	molecular_function	identical protein binding
G	0043596	cellular_component	nuclear replication fork
G	0043626	cellular_component	PCNA complex
G	0044849	biological_process	estrous cycle
G	0044877	molecular_function	protein-containing complex binding
G	0045739	biological_process	positive regulation of DNA repair
G	0045740	biological_process	positive regulation of DNA replication
G	0046686	biological_process	response to cadmium ion
G	0070062	cellular_component	extracellular exosome
G	0070182	molecular_function	DNA polymerase binding
G	0070301	biological_process	cellular response to hydrogen peroxide
G	0070557	cellular_component	PCNA-p21 complex
G	0071548	biological_process	response to dexamethasone
G	0097421	biological_process	liver regeneration
G	0140861	biological_process	DNA repair-dependent chromatin remodeling
G	1902065	biological_process	response to L-glutamate
G	1902969	biological_process	mitotic DNA replication
G	1902990	biological_process	mitotic telomere maintenance via semi-conservative replication

Functional Information from PROSITE/UniProt

site_id	PS00293
Number of Residues	19
Details	PCNA_2 Proliferating cell nuclear antigen signature 2. RCDRnlaMgvnLtSMsKIL

Chain	Residue	Details
E	ARG61-LEU79

---

site_id	PS01251
Number of Residues	24
Details	PCNA_1 Proliferating cell nuclear antigen signature 1. GVnLqSMDsSHVsLVqLtLrsegF

Chain	Residue	Details
E	GLY34-PHE57

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	112
Details	Domain: {"description":"VRR-NUC"}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	25
Details	Coiled coil: {"evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"Q9I2N0","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	57
Details	DNA binding: {"evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	279
Details	Region: {"description":"Interaction with NUDT15","evidences":[{"source":"PubMed","id":"19419956","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	3
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19419956","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	9
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	3
Details	Modified residue: {"description":"Phosphotyrosine; by EGFR","evidences":[{"source":"PubMed","id":"17115032","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38459011","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	3
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate","evidences":[{"source":"PubMed","id":"17108083","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17130289","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	4
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---