9FG9
Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Etomidate in the long-lived symmetric desensitised state
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D (A, D) | Gamma-aminobutyric acid receptor subunit alpha-1 | polymer | 464 | 52916.6 | 2 | UniProt (P14867) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (human) | GABA(A) receptor subunit alpha-1 |
| 2 | B, E (B, E) | Gamma-aminobutyric acid receptor subunit beta-3 | polymer | 473 | 54444.6 | 2 | UniProt (P28472) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (human) | GABA(A) receptor subunit beta-3 |
| 3 | C (C) | Gamma-aminobutyric acid receptor subunit gamma-2 | polymer | 495 | 56922.1 | 1 | UniProt (P18507) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (human) | GABA(A) receptor subunit gamma-2 |
| 4 | F (a) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1721.5 | 1 | In PDB GlyTouCan (G40702WU) | |||
| 5 | G, I, K (b, c, e) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 3 | In PDB GlyTouCan (G42666HT) | |||
| 6 | H, L (F, G) | alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1073.0 | 2 | In PDB GlyTouCan (G09724ZC) | |||
| 7 | J (d) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 1 | In PDB GlyTouCan (G22768VO) | |||
| 8 | M, U, W (A, C, D) | CHLORIDE ION | non-polymer | 35.5 | 3 | Chemie (CL) | |||
| 9 | N, X (A, D) | [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate | non-polymer | 746.6 | 2 | Chemie (PIO) | |||
| 10 | AA, O, R, V, Y (E, A, B, C, D) | DECANE | non-polymer | 142.3 | 5 | Chemie (D10) | |||
| 11 | P, Z (A, D) | 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine | non-polymer | 760.1 | 2 | Chemie (LBN) | |||
| 12 | DA, Q (E, B) | HEXADECANE | non-polymer | 226.4 | 2 | Chemie (R16) | |||
| 13 | BA, S (E, B) | GAMMA-AMINO-BUTANOIC ACID | non-polymer | 103.1 | 2 | Chemie (ABU) | |||
| 14 | CA, T (E, B) | Etomidate | non-polymer | 244.3 | 2 | Chemie (V8D) | |||
| 15 | EA, FA, GA, HA, IA (A, B, C, D, E) | water | water | 18.0 | 172 | Chemie (HOH) |
Sequence modifications
A, D: 1 - 429 (UniProt: P14867)
C: -38 - 436 (UniProt: P18507)
| PDB | External Database | Details |
|---|---|---|
| Met -34 | - | initiating methionine |
| Lys -33 | - | expression tag |
| Lys -32 | - | expression tag |
| Ser -31 | - | expression tag |
| Pro -30 | - | expression tag |
| Gly -29 | - | expression tag |
| Leu -28 | - | expression tag |
| Ser -27 | - | expression tag |
| Asp -26 | - | expression tag |
| Tyr -25 | - | expression tag |
| Leu -24 | - | expression tag |
| Trp -23 | - | expression tag |
| Ala -22 | - | expression tag |
| Trp -21 | - | expression tag |
| Thr -20 | - | expression tag |
| Leu -19 | - | expression tag |
| Phe -18 | - | expression tag |
| Leu -17 | - | expression tag |
| Ser -16 | - | expression tag |
| Thr -15 | - | expression tag |
| Leu -14 | - | expression tag |
| Thr -13 | - | expression tag |
| Gly -12 | - | expression tag |
| Arg -11 | - | expression tag |
| Ser -10 | - | expression tag |
| Tyr -9 | - | expression tag |
| Gly -8 | - | expression tag |
| Asp -7 | - | expression tag |
| Tyr -6 | - | expression tag |
| Lys -5 | - | expression tag |
| Asp -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Lys 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 437 | - | expression tag |
| Gly 438 | - | expression tag |
| Ser 439 | - | expression tag |
| Gly 440 | - | expression tag |
| Gly 441 | - | expression tag |
| Ser 442 | - | expression tag |
| Gly 443 | - | expression tag |
| Gly 444 | - | expression tag |
| Ser 445 | - | expression tag |
| Gly 446 | - | expression tag |
| Lys 447 | - | expression tag |
| Thr 448 | - | expression tag |
| Glu 449 | - | expression tag |
| Thr 450 | - | expression tag |
| Ser 451 | - | expression tag |
| Gln 452 | - | expression tag |
| Val 453 | - | expression tag |
| Ala 454 | - | expression tag |
| Pro 455 | - | expression tag |
| Ala 456 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 271644.4 | |
| Branched | Number of molecules | 7 |
| Total formula weight | 6051.5 | |
| Non-Polymers* | Number of molecules | 18 |
| Total formula weight | 4978.8 | |
| All* | Total formula weight | 282674.6 |
