9FAX
CryoEM structure of human full-length beta3gamma2 GABA(A) receptor in complex with Megabody25, doubly occupied GARLH4 and Neuroligin2 TMD, in a closed state
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, G (E, C) | Isoform 2 of Gamma-aminobutyric acid receptor subunit gamma-2 | polymer | 405 | 47870.4 | 2 | UniProt (P18507) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (human) | GABA(A) receptor subunit gamma-2,GABAAR subunit gamma-2 |
| 2 | B, H (G, H) | Neuroligin-2 | polymer | 33 | 3614.1 | 2 | UniProt (Q8NFZ4) | Homo sapiens (human) | |
| 3 | C, I (I, L) | LHFPL tetraspan subfamily member 4 protein | polymer | 188 | 20634.4 | 2 | UniProt (Q7Z7J7) Pfam (PF10242) | Homo sapiens (human) | GABAA receptor regulatory Lhfpl4,Lipoma HMGIC fusion partner-like 4 protein |
| 4 | D, E, F (B, A, D) | Gamma-aminobutyric acid receptor subunit beta-3 | polymer | 439 | 50733.4 | 3 | UniProt (P28472) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (human) | GABA(A) receptor subunit beta-3,GABAAR subunit beta-3 |
| 5 | J (F) | Megabody25 | polymer | 522 | 56300.6 | 1 | Lama glama | ||
| 6 | K (J) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1235.1 | 1 | In PDB GlyTouCan (G55220VL) | |||
| 7 | L, N (K, N) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 2 | In PDB GlyTouCan (G15407YE) | |||
| 8 | M (M) | alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1073.0 | 1 | In PDB GlyTouCan (G09724ZC) | |||
| 9 | O (O) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G42666HT) | |||
| 10 | P (P) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 1 | In PDB GlyTouCan (G22768VO) | |||
| 11 | Q, TA (E, C) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 2 | Chemie (NAG) | |||
| 12 | R, S, UA, V, VA (E, C) | HEXADECANE | non-polymer | 226.4 | 5 | Chemie (R16) | |||
| 13 | T, Y (E, B) | 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE | non-polymer | 535.7 | 2 | Chemie (PX2) | |||
| 14 | U, ZA (E, C) | CHOLESTEROL | non-polymer | 386.7 | 2 | Chemie (CLR) | |||
| 15 | AB, BB, W, X (C, L, I) | (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate | non-polymer | 749.0 | 4 | Chemie (PGW) | |||
| 16 | AA, CA, FA, GA, HA... (B, A, D, C) | HEXANE | non-polymer | 86.2 | 13 | Chemie (HEX) | |||
| 17 | BA, DA, KA, MA, NA (B, D) | DECANE | non-polymer | 142.3 | 5 | Chemie (D10) | |||
| 18 | EA, LA, QA, WA, XA (A, D, C) | PALMITIC ACID | non-polymer | 256.4 | 5 | Chemie (PLM) |
Sequence modifications
E, C: 25 - 428 (UniProt: P18507)
| PDB | External Database | Details |
|---|---|---|
| Gly 429 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 352738.6 | |
| Branched | Number of molecules | 6 |
| Total formula weight | 4816.4 | |
| Non-Polymers* | Number of molecules | 38 |
| Total formula weight | 9529.1 | |
| All* | Total formula weight | 367084.1 |
