9F34

Cryo-EM structure of Dopamine 3 receptor:Go complex bound to bitopic FOB02-04A - Conformation B

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(o) subunit alpha	polymer	354	40097.5	1	UniProt (P09471) Pfam (PF00503) In PDB	Homo sapiens (human)
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	355	39374.0	1	UniProt (P54311) Pfam (PF00400) In PDB	Rattus norvegicus (Norway rat)	Transducin beta chain 1
3	C	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7845.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
4	E	Antibody scFv16	polymer	259	27625.8	1		Mus musculus (house mouse)
5	R	Green fluorescent protein,D(3) dopamine receptor	polymer	669	74733.9	1	UniProt (P42212) UniProt (P35462) Pfam (PF01353) Pfam (PF00001) In PDB	Homo sapiens (human)	Dopamine D3 receptor
6	R	N-[2-[(1R,2S)-2-[[(2S,5S)-2-(6-azanylpyridin-3-yl)-5-methyl-morpholin-4-yl]methyl]cyclopropyl]ethyl]-1H-indole-2-carboxamide	non-polymer		433.5	1	Chemie (A1H9N)

Sequence modifications

A: 1 - 181 (UniProt: P09471)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation

A: 182 - 354 (UniProt: P09471)

PDB	External Database	Details
Ala 204	Gly 204	engineered mutation
Ala 246	Glu 246	engineered mutation
Lys 249	Met 249	engineered mutation
Ser 326	Ala 326	engineered mutation

B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -14	-	initiating methionine
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
Glu -5	-	expression tag
Asn -4	-	expression tag
Leu -3	-	expression tag
Tyr -2	-	expression tag
Phe -1	-	expression tag
Gln 0	-	expression tag
Gly 1	-	expression tag

C: 1 - 71 (UniProt: P59768)

PDB	External Database	Details
Ser 68	Cys 68	engineered mutation

R: -243 - -7 (UniProt: P42212)

PDB	External Database	Details
Met -268	-	initiating methionine
Lys -267	-	expression tag
Thr -266	-	expression tag
Ile -265	-	expression tag
Ile -264	-	expression tag
Ala -263	-	expression tag
Leu -262	-	expression tag
Ser -261	-	expression tag
Tyr -260	-	expression tag
Ile -259	-	expression tag
Phe -258	-	expression tag
Cys -257	-	expression tag
Leu -256	-	expression tag
Val -255	-	expression tag
Phe -254	-	expression tag
Ala -253	-	expression tag
Asp -252	-	expression tag
Tyr -251	-	expression tag
Lys -250	-	expression tag
Asp -249	-	expression tag
Asp -248	-	expression tag
Asp -247	-	expression tag
Asp -246	-	expression tag
Lys -245	-	expression tag
Val -244	-	expression tag
Leu -181	Phe 64	engineered mutation
Thr -180	Ser 65	engineered mutation
Leu -14	His 231	engineered mutation
Leu -6	-	linker
Glu -5	-	linker
Val -4	-	linker
Leu -3	-	linker
Phe -2	-	linker
Gln -1	-	linker
Gly 0	-	linker
Pro 1	-	linker

R: 2 - 31 (UniProt: P35462)

PDB	External Database	Details
Ser 9	Gly 9	conflict

R: 32 - 322 (UniProt: P35462)

PDB	External Database	Details
Trp 119	Leu 119	engineered mutation

R: 323 - 400 (UniProt: P35462)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	189676.4
Non-Polymers*	Number of molecules	1
	Total formula weight	433.5
All*	Total formula weight	190109.9

*Water molecules are not included.