9EN2
Crystal structure of the metalloproteinase enhancer PCPE-1 complexed with nanobodies VHH-H4 and VHH-I5
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Procollagen C-endopeptidase enhancer 1 | polymer | 302 | 32437.4 | 1 | UniProt (Q15113) Pfam (PF00431) | Homo sapiens (human) | Procollagen COOH-terminal proteinase enhancer 1,PCPE-1,Procollagen C-proteinase enhancer 1,Type 1 procollagen C-proteinase enhancer protein,Type I procollagen COOH-terminal proteinase enhancer |
| 2 | B (B) | VHH-H4 | polymer | 154 | 16608.4 | 1 | Lama glama | ||
| 3 | C (C) | VHH-I5 | polymer | 144 | 15628.6 | 1 | Lama glama | ||
| 4 | D, E (A) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 5 | F, G (B, C) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 6 | H, I, J (A, B, C) | water | water | 18.0 | 365 | Chemie (HOH) |
Sequence modifications
A: 25 - 277 (UniProt: Q15113)
| PDB | External Database | Details |
|---|---|---|
| Met -21 | - | initiating methionine |
| Gly -20 | - | expression tag |
| Ile -19 | - | expression tag |
| Leu -18 | - | expression tag |
| Pro -17 | - | expression tag |
| Ser -16 | - | expression tag |
| Pro -15 | - | expression tag |
| Gly -14 | - | expression tag |
| Met -13 | - | expression tag |
| Pro -12 | - | expression tag |
| Ala -11 | - | expression tag |
| Leu -10 | - | expression tag |
| Leu -9 | - | expression tag |
| Ser -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Val -6 | - | expression tag |
| Ser -5 | - | expression tag |
| Leu -4 | - | expression tag |
| Leu -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Val -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Leu 1 | - | expression tag |
| Met 2 | - | expression tag |
| Gly 3 | - | expression tag |
| Cys 4 | - | expression tag |
| Val 5 | - | expression tag |
| Ala 6 | - | expression tag |
| Glu 7 | - | expression tag |
| Thr 8 | - | expression tag |
| Gly 9 | - | expression tag |
| His 10 | - | expression tag |
| His 11 | - | expression tag |
| His 12 | - | expression tag |
| His 13 | - | expression tag |
| His 14 | - | expression tag |
| His 15 | - | expression tag |
| Leu 16 | - | expression tag |
| Glu 17 | - | expression tag |
| Val 18 | - | expression tag |
| Leu 19 | - | expression tag |
| Phe 20 | - | expression tag |
| Gln 21 | - | expression tag |
| Gly 22 | - | expression tag |
| Pro 23 | - | expression tag |
| Ala 24 | - | expression tag |
| Ala 31 | Thr 31 | conflict |
| Leu 278 | - | expression tag |
| Gly 279 | - | expression tag |
| Thr 280 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 64674.5 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 264.3 | |
| All* | Total formula weight | 64938.9 |
