9CQ3

The gap-filling complex with Pol mu engaged in the NHEJ pathway

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, N (A, a)	X-ray repair cross-complementing protein 6	polymer	612	70198.3	2	UniProt (P12956) Pfam (PF03731) Pfam (PF02735) Pfam (PF03730) Pfam (PF02037)	Homo sapiens (human)	5'-deoxyribose-5-phosphate lyase Ku70,5'-dRP lyase Ku70,70 kDa subunit of Ku antigen,ATP-dependent DNA helicase 2 subunit 1,ATP-dependent DNA helicase II 70 kDa subunit,CTC box-binding factor 75 kDa subunit,CTCBF,DNA repair protein XRCC6,Lupus Ku autoantigen protein p70,Ku70,Thyroid-lupus autoantigen,TLAA,X-ray repair complementing defective repair in Chinese hamster cells 6
2	B, O (B, b)	X-ray repair cross-complementing protein 5	polymer	732	82812.4	2	UniProt (P13010) Pfam (PF03731) Pfam (PF02735) Pfam (PF03730) Pfam (PF08785)	Homo sapiens (human)
3	C, P (C, c)	Non-homologous end-joining factor 1	polymer	302	33625.5	2	UniProt (Q9H9Q4) Pfam (PF21928) Pfam (PF09302)	Homo sapiens (human)	Protein cernunnos,XRCC4-like factor
4	D, E, Q, R (D, E, d, e)	DNA repair protein XRCC4	polymer	336	38337.7	4	UniProt (Q13426) Pfam (PF06632) Pfam (PF21924) Pfam (PF21925)	Homo sapiens (human)	X-ray repair cross-complementing protein 4
5	F, S (F, f)	DNA ligase 4	polymer	914	104378.3	2	UniProt (P49917) Pfam (PF04675) Pfam (PF01068) Pfam (PF04679) Pfam (PF00533) Pfam (PF11411)	Homo sapiens (human)	DNA ligase IV,Polydeoxyribonucleotide synthase [ATP] 4
6	G, H (G, H)	Protein PAXX	polymer	218	23282.2	2	UniProt (Q9BUH6) Pfam (PF15384)	Homo sapiens (human)	Paralog of XRCC4 and XLF,XRCC4-like small protein
7	I (I)	DNA (38-MER)	polymer	68	20737.3	1		Homo sapiens
8	J (J)	DNA (42-MER)	polymer	68	20795.4	1		Homo sapiens
9	K (K)	DNA (34-MER)	polymer	51	15865.2	1		Homo sapiens
10	L (L)	DNA (37-MER)	polymer	50	15493.9	1		Homo sapiens
11	M, T (M, m)	DNA-directed DNA/RNA polymerase mu	polymer	512	56973.7	2	UniProt (Q9NP87) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791)	Homo sapiens (human)	Pol Mu,Terminal transferase
12	U, V (M)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
13	W (M)	2'-deoxy-5'-O-[(R)-hydroxy{[(R)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]adenosine	non-polymer		490.2	1	Chemie (DZ4)

Sequence modifications

A, a: 1 - 609 (UniProt: P12956)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
Val 0	-	expression tag

C, c: 1 - 299 (UniProt: Q9H9Q4)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
Val 0	-	expression tag

F, f: 1 - 911 (UniProt: P49917)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
Val 0	-	expression tag

G, H: 1 - 422 (UniProt: Q9BUH6)

PDB	External Database	Details
Met -13	-	expression tag
Gly -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Gln -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag

M, m: 1 - 494 (UniProt: Q9NP87)

PDB	External Database	Details
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
Gly -9	-	expression tag
Leu -8	-	expression tag
Glu -7	-	expression tag
Val -6	-	expression tag
Leu -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	20
	Total formula weight	968783.4
Non-Polymers*	Number of molecules	3
	Total formula weight	538.8
All*	Total formula weight	969322.2

*Water molecules are not included.