9C5A

AP-3 Arf1 dimeric interface, focused refinement

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (B, b)	AP-3 complex subunit beta-1	polymer	684	77286.5	2	UniProt (O00203) Pfam (PF01602)	Homo sapiens (human)	Adaptor protein complex AP-3 subunit beta-1,Adaptor-related protein complex 3 subunit beta-1,Beta-3A-adaptin,Clathrin assembly protein complex 3 beta-1 large chain
2	B, F (M, m)	AP-3 complex subunit mu-1	polymer	418	46990.0	2	UniProt (Q9Y2T2) Pfam (PF01217) Pfam (PF00928)	Homo sapiens (human)	AP-3 adaptor complex mu3A subunit,Adaptor-related protein complex 3 subunit mu-1,Mu-adaptin 3A,Mu3A-adaptin
3	C, G (C, c)	ADP-ribosylation factor 1	polymer	182	20775.8	2	UniProt (P84077) Pfam (PF00025)	Homo sapiens (human)
4	D, H (Y, y)	Lysosome-associated membrane glycoprotein 1	polymer	12	1377.6	2	UniProt (P11279)	Homo sapiens	LAMP-1,Lysosome-associated membrane protein 1,CD107 antigen-like family member A
5	I, K (C, c)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	J, L (C, c)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)

Sequence modifications

B, b: 1 - 677 (UniProt: O00203)

PDB	External Database	Details
Gly 678	-	expression tag
Leu 679	-	expression tag
Glu 680	-	expression tag
Val 681	-	expression tag
Leu 682	-	expression tag
Phe 683	-	expression tag
Gln 684	-	expression tag

C, c: 2 - 181 (UniProt: P84077)

PDB	External Database	Details
Leu 71	Gln 71	engineered mutation
Ser 182	-	expression tag
Leu 183	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	292859.7
Non-Polymers*	Number of molecules	4
	Total formula weight	1095.0
All*	Total formula weight	293954.7

*Water molecules are not included.