9C5A
AP-3 Arf1 dimeric interface, focused refinement
EM specimen
| Vitrification | Yes |
| Instrument | SPOTITON |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.2 |
| Software | cryoSPARC,Rosetta,PHENIX,Leginon,UCSF ChimeraX |
| Symmetry | C2 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
