9C50
Replacement of a single residue changes the primary specificity of thrombin
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, C (A, C) | Thrombin A-chain | polymer | 36 | 4096.5 | 2 | UniProt (P00734) Pfam (PF09396) | Homo sapiens (human) | Coagulation factor II |
2 | B, D (B, D) | Thrombin B-chain | polymer | 273 | 31425.0 | 2 | UniProt (P00734) Pfam (PF00089) | Homo sapiens (human) | |
3 | E, F (E, F) | FPF | polymer | 3 | 441.9 | 2 | synthetic construct | ||
4 | G, H (B, D) | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
5 | I, J, K, L (A, B, C, D) | water | water | 18.0 | 121 | Chemie (HOH) |
Sequence modifications
B, D: 16 - 247 (UniProt: P00734)
PDB | External Database | Details |
---|---|---|
Tyr 248 | - | expression tag |
Leu 249 | - | expression tag |
Glu 250 | - | expression tag |
Asp 251 | - | expression tag |
Gln 252 | - | expression tag |
Val 253 | - | expression tag |
Asp 254 | - | expression tag |
Pro 255 | - | expression tag |
Arg 256 | - | expression tag |
Leu 257 | - | expression tag |
Ile 258 | - | expression tag |
Asp 259 | - | expression tag |
Gly 260 | - | expression tag |
Lys 261 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 71927.0 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 46.0 | |
All* | Total formula weight | 71973.0 |