9C1B

Crystal structure of GDP-bound human M-RAS protein in crystal form II

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Ras-related protein M-Ras	polymer	219	25324.9	4	UniProt (O14807) Pfam (PF00071)	Homo sapiens (human)	Ras-related protein R-Ras3
2	E, I, L, P (A, B, C, D)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
3	F, J, M, Q (A, B, C, D)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	4	Chemie (GDP)
4	G, K (A, B)	TRIETHYLENE GLYCOL	non-polymer		150.2	2	Chemie (PGE)
5	H, O (A, C)	TETRAETHYLENE GLYCOL	non-polymer		194.2	2	Chemie (PG4)
6	N (C)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
7	R, S, T, U (A, B, C, D)	water	water		18.0	351	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 204 (UniProt: O14807)

PDB	External Database	Details
Met -14	-	initiating methionine
Gly -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	101299.7
Non-Polymers*	Number of molecules	13
	Total formula weight	2664.9
All*	Total formula weight	103964.7

*Water molecules are not included.