9C1B

Crystal structure of GDP-bound human M-RAS protein in crystal form II

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Ras-related protein M-Ras	polymer	219	25324.9	4	UniProt (O14807) Pfam (PF00071)	Homo sapiens (human)	Ras-related protein R-Ras3
2	E, I, L, P (A, B, C, D)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG) PubChem (888)
3	F, J, M, Q (A, B, C, D)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	4	Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537)
4	G, K (A, B)	TRIETHYLENE GLYCOL	non-polymer		150.2	2	Chemie (PGE)
5	H, O (A, C)	TETRAETHYLENE GLYCOL	non-polymer		194.2	2	Chemie (PG4) PubChem (8200)
6	N (C)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG) PubChem (8117)
7	R, S, T, U (A, B, C, D)	water	water		18.0	351	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 204 (UniProt: O14807)

PDB	External Database	Details
Met -14	-	initiating methionine
Gly -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	101299.7
Non-Polymers*	Number of molecules	13
	Total formula weight	2664.9
All*	Total formula weight	103964.7

*Water molecules are not included.