9C1B

Crystal structure of GDP-bound human M-RAS protein in crystal form II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	53.724, 55.604, 67.770
Unit cell angles	103.74, 90.14, 93.64

Refinement procedure

Resolution	41.940 - 2.270
R-factor	0.2021
Rwork	0.199
R-free	0.24690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.527
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.940	2.351
High resolution limit [Å]	2.270	2.270
Rmeas	0.087	0.363
Rpim	0.049	0.207
Number of reflections	32348	3405
<I/σ(I)>	11.81	3.71
Completeness [%]	91.3
Redundancy	2.7
CC(1/2)	0.985	0.708

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	20% PEG3350