9BJU

Crystal structure of the complex between VHL, ElonginB, ElonginC, and compound 5

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, G, J	Elongin-B	polymer	104	11748.4	4	UniProt (Q15370) Pfam (PF00240) In PDB	Homo sapiens (human)	EloB,Elongin 18 kDa subunit,RNA polymerase II transcription factor SIII subunit B,SIII p18,Transcription elongation factor B polypeptide 2
2	B, E, H, K	Elongin-C	polymer	96	10843.4	4	UniProt (Q15369) Pfam (PF03931) In PDB	Homo sapiens (human)	EloC, Elongin 15 kDa subunit, RNA polymerase II transcription factor SIII subunit C, SIII p15, Transcription elongation factor B polypeptide 1
3	C, L	von Hippel-Lindau disease tumor suppressor	polymer	176	20530.3	2	UniProt (P40337) Pfam (PF01847) Pfam (PF17211) In PDB	Homo sapiens (human)	Protein G7,pVHL
4	F, I	von Hippel-Lindau disease tumor suppressor	polymer	176	20514.3	2	UniProt (P40337) Pfam (PF01847) Pfam (PF17211) In PDB	Homo sapiens (human)	Protein G7,pVHL
5	W, X, Y, Z	3-PYRIDIN-4-YL-2,4-DIHYDRO-INDENO[1,2-.C.]PYRAZOLE	polymer	5	491.6	4		synthetic construct
6	B, K	trifluoroacetic acid	non-polymer		114.0	2	Chemie (TFA)
7	B	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
8	C, K	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
9	C, F, H	ACETATE ION	non-polymer		59.0	3	Chemie (ACT)
10	L, H, I	DIMETHYL SULFOXIDE	non-polymer		78.1	3	Chemie (DMS)
11		water	water		18.0	128	Chemie (HOH)

Sequence modifications

C, L: 54 - 213 (UniProt: P40337)

PDB	External Database	Details
Met 38	-	initiating methionine
His 39	-	expression tag
His 40	-	expression tag
His 41	-	expression tag
His 42	-	expression tag
His 43	-	expression tag
His 44	-	expression tag
Gly 45	-	expression tag
Glu 46	-	expression tag
Asn 47	-	expression tag
Leu 48	-	expression tag
Tyr 49	-	expression tag
Phe 50	-	expression tag
Gln 51	-	expression tag
Gly 52	-	expression tag
Ser 53	-	expression tag

F, I: 54 - 213 (UniProt: P40337)

PDB	External Database	Details
Met 38	-	initiating methionine
His 39	-	expression tag
His 40	-	expression tag
His 41	-	expression tag
His 42	-	expression tag
His 43	-	expression tag
His 44	-	expression tag
Gly 45	-	expression tag
Glu 46	-	expression tag
Asn 47	-	expression tag
Leu 48	-	expression tag
Tyr 49	-	expression tag
Phe 50	-	expression tag
Gln 51	-	expression tag
Gly 52	-	expression tag
Ser 53	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	174422.8
Non-Polymers*	Number of molecules	11
	Total formula weight	869.8
All*	Total formula weight	175292.6

*Water molecules are not included.