9BJU

Crystal structure of the complex between VHL, ElonginB, ElonginC, and compound 5

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	93
Detector technology	PIXEL
Collection date	2018-05-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97893
Spacegroup name	P 41 2 2
Unit cell lengths	93.297, 93.297, 362.757
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.860 - 2.470
R-factor	0.2203
Rwork	0.218
R-free	0.25565
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.374
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.560
High resolution limit [Å]	2.470	5.320	2.470
Rmerge	0.136	0.074	1.228
Rmeas	0.142	0.078	1.281
Rpim	0.040	0.023	0.358
Number of reflections	58657	6328	5725
<I/σ(I)>	4.5
Completeness [%]	99.9	99.6	100
Redundancy	13.1	12.3	12.5
CC(1/2)		0.989	0.839

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 mM sodium cacodylate pH 5.7, 15% PEG8000, 0.2 M magnesium acetate, 5 mM DTT