9B12

Structure of Optineurin bound to HOIP NZF1 domain and M1-linked diubiquitin, crystal form 1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (C, D, E, F)	Optineurin	polymer	96	11017.5	4	UniProt (Q96CV9) Pfam (PF16516)	Homo sapiens (human)	E3-14.7K-interacting protein,FIP-2,Huntingtin yeast partner L,Huntingtin-interacting protein 7,HIP-7,Huntingtin-interacting protein L,NEMO-related protein,Optic neuropathy-inducing protein,Transcription factor IIIA-interacting protein,TFIIIA-IntP
2	E, F (A, B)	Ubiquitin	polymer	152	17135.7	2	UniProt (P0CG47) Pfam (PF00240)	Homo sapiens (human)
3	G, H, I, J (G, H, I, J)	E3 ubiquitin-protein ligase RNF31	polymer	30	3316.8	4	UniProt (Q96EP0)	Homo sapiens (human)	HOIL-1-interacting protein,HOIP,RING finger protein 31,RING-type E3 ubiquitin transferase RNF31,Zinc in-between-RING-finger ubiquitin-associated domain protein
4	K, V (C, J)	TETRAETHYLENE GLYCOL	non-polymer		194.2	2	Chemie (PG4)
5	L (C)	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
6	M, P, W (F, B, J)	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
7	N, O, S, X (F, A, H, J)	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	4	Chemie (EPE)
8	Q, R, T, U (G, H, I, J)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
9	AA, BA, CA, DA, EA... (E, F, A, B, G...)	water	water		18.0	187	Chemie (HOH)

Sequence modifications

C, D, E, F: 419 - 512 (UniProt: Q96CV9)

PDB	External Database	Details
Gly 417	-	expression tag
Pro 418	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	91608.5
Non-Polymers*	Number of molecules	14
	Total formula weight	1859.8
All*	Total formula weight	93468.4

*Water molecules are not included.