9B12
Structure of Optineurin bound to HOIP NZF1 domain and M1-linked diubiquitin, crystal form 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.96863 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 110.413, 70.191, 193.944 |
| Unit cell angles | 90.00, 102.25, 90.00 |
Refinement procedure
| Resolution | 46.150 - 1.810 |
| R-factor | 0.2331 |
| Rwork | 0.231 |
| R-free | 0.26850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.660 | 2.140 |
| High resolution limit [Å] | 1.810 | 2.100 |
| Rmerge | 0.074 | 0.697 |
| Rmeas | 0.089 | 0.840 |
| Rpim | 0.050 | 0.464 |
| Total number of observations | 13825 | |
| Number of reflections | 84203 | 4419 |
| <I/σ(I)> | 6.6 | 1.1 |
| Completeness [%] | 99.7 | |
| Redundancy | 3.1 | 3.1 |
| CC(1/2) | 0.995 | 0.682 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 3:1 with reservoir solution containing 50% PEG 200 and 0.1 M HEPES pH 7.5 |
