9AXF
Structure of human calcium-sensing receptor in complex with chimeric Gq (miniGisq) protein in detergent
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (R, Q) | Extracellular calcium-sensing receptor | polymer | 911 | 102864.6 | 2 | UniProt (P41180) Pfam (PF01094) Pfam (PF07562) Pfam (PF00003) | Homo sapiens (human) | CaR,CaSR,hCasR,Parathyroid cell calcium-sensing receptor 1,PCaR1 |
| 2 | C (A) | Guanine nucleotide-binding protein G(i) subunit alpha-1,Adenylate cyclase-stimulating G alpha protein | polymer | 246 | 28306.1 | 1 | UniProt (P63096) UniProt (A0A590UJY2) Pfam (PF00503) UniProt (by SIFTS) (P63092) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 3 | D (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 348 | 38413.9 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1 |
| 4 | E (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 5 | F (H) | Single-chain antibody fragment scFv16 | polymer | 297 | 31870.6 | 1 | Mus musculus | ||
| 6 | G (N) | Nanobody Nb-35 | polymer | 160 | 17352.5 | 1 | Lama glama | ||
| 7 | H, I, J, K (C, D, E, F) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 4 | In PDB GlyTouCan (G42666HT) | |||
| 8 | CA, DA, EA, FA, L... (Q, R) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 8 | Chemie (NAG) | |||
| 9 | GA, P (Q, R) | CYCLOMETHYLTRYPTOPHAN | non-polymer | 216.2 | 2 | Chemie (TCR) | |||
| 10 | HA, Q (Q, R) | PHOSPHATE ION | non-polymer | 95.0 | 2 | Chemie (PO4) | |||
| 11 | IA, JA, KA, LA, R... (Q, R) | CALCIUM ION | non-polymer | 40.1 | 7 | Chemie (CA) | |||
| 12 | MA, U (Q, R) | 3-(2-chlorophenyl)-N-[(1R)-1-(3-methoxyphenyl)ethyl]propan-1-amine | non-polymer | 303.8 | 2 | Chemie (9IG) | |||
| 13 | V (R) | Lauryl Maltose Neopentyl Glycol | non-polymer | 1005.2 | 1 | Chemie (AV0) | |||
| 14 | NA, W, X, Y (Q, R) | CHOLESTEROL HEMISUCCINATE | non-polymer | 486.7 | 4 | Chemie (Y01) | |||
| 15 | AA, BA, OA, PA, QA... (R, Q) | CHOLESTEROL | non-polymer | 386.7 | 8 | Chemie (CLR) |
Sequence modifications
R, Q: 1 - 903 (UniProt: P41180)
A: 1 - 53 (UniProt: P63096)
A: 69 - 246 (UniProt: A0A590UJY2)
B: 2 - 340 (UniProt: P62873)
| PDB | External Database | Details |
|---|---|---|
| Asp 904 | - | expression tag |
| Tyr 905 | - | expression tag |
| Lys 906 | - | expression tag |
| Asp 907 | - | expression tag |
| Asp 908 | - | expression tag |
| Asp 909 | - | expression tag |
| Asp 910 | - | expression tag |
| Lys 911 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Glu 20 | Asp 20 | engineered mutation |
| Lys 21 | Arg 21 | engineered mutation |
| Gln 22 | Asn 22 | engineered mutation |
| Gln 24 | Arg 24 | engineered mutation |
| Lys 25 | Glu 25 | engineered mutation |
| Lys 27 | Gly 27 | engineered mutation |
| Gln 28 | Glu 28 | engineered mutation |
| Val 29 | Lys 29 | engineered mutation |
| Tyr 30 | Ala 30 | engineered mutation |
| Arg 31 | Ala 31 | engineered mutation |
| Ala 32 | Arg 32 | engineered mutation |
| Thr 33 | Glu 33 | engineered mutation |
| His 34 | Val 34 | engineered mutation |
| Arg 35 | Lys 35 | engineered mutation |
| Asp 42 | Gly 42 | engineered mutation |
| Asn 43 | Glu 43 | engineered mutation |
| Arg 54 | - | linker |
| Ile 55 | - | linker |
| Leu 56 | - | linker |
| His 57 | - | linker |
| Gly 58 | - | linker |
| Gly 59 | - | linker |
| Ser 60 | - | linker |
| Gly 61 | - | linker |
| Gly 62 | - | linker |
| Ser 63 | - | linker |
| Gly 64 | - | linker |
| Gly 65 | - | linker |
| Thr 66 | - | linker |
| Ser 67 | - | linker |
| Gly 68 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Asp 111 | Ala 92 | engineered mutation |
| Asp 114 | Ser 95 | engineered mutation |
| - | Asn 97 | deletion |
| - | Met 98 | deletion |
| - | Val 99 | deletion |
| - | Ile 100 | deletion |
| - | Arg 101 | deletion |
| - | Glu 102 | deletion |
| - | Asp 103 | deletion |
| - | Asn 104 | deletion |
| - | Gln 105 | deletion |
| - | Thr 106 | deletion |
| Asp 124 | Leu 115 | engineered mutation |
| Ala 224 | Ile 215 | engineered mutation |
| Ile 227 | Val 218 | engineered mutation |
| Lys 232 | Arg 223 | engineered mutation |
| Leu 236 | Gln 227 | engineered mutation |
| Gln 237 | Arg 228 | engineered mutation |
| Asn 239 | His 230 | engineered mutation |
| Glu 242 | Gln 233 | engineered mutation |
| Asn 244 | Glu 235 | engineered mutation |
| Val 246 | Leu 237 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -7 | - | initiating methionine |
| Asp -6 | - | expression tag |
| Tyr -5 | - | expression tag |
| Lys -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Asp 0 | - | expression tag |
| Lys 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 7 |
| Total formula weight | 329533.5 | |
| Branched | Number of molecules | 4 |
| Total formula weight | 1697.6 | |
| Non-Polymers* | Number of molecules | 34 |
| Total formula weight | 9325.6 | |
| All* | Total formula weight | 340556.7 |
