9AXF
Structure of human calcium-sensing receptor in complex with chimeric Gq (miniGisq) protein in detergent
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003924 | molecular_function | GTPase activity |
| A | 0005525 | molecular_function | GTP binding |
| A | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| A | 0019001 | molecular_function | guanyl nucleotide binding |
| A | 0031683 | molecular_function | G-protein beta/gamma-subunit complex binding |
| B | 0001750 | cellular_component | photoreceptor outer segment |
| B | 0003924 | molecular_function | GTPase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005765 | cellular_component | lysosomal membrane |
| B | 0005829 | cellular_component | cytosol |
| B | 0005834 | cellular_component | heterotrimeric G-protein complex |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0007165 | biological_process | signal transduction |
| B | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| B | 0007191 | biological_process | adenylate cyclase-activating dopamine receptor signaling pathway |
| B | 0007200 | biological_process | phospholipase C-activating G protein-coupled receptor signaling pathway |
| B | 0007213 | biological_process | G protein-coupled acetylcholine receptor signaling pathway |
| B | 0007265 | biological_process | Ras protein signal transduction |
| B | 0008283 | biological_process | cell population proliferation |
| B | 0016020 | cellular_component | membrane |
| B | 0030159 | molecular_function | signaling receptor complex adaptor activity |
| B | 0044877 | molecular_function | protein-containing complex binding |
| B | 0045202 | cellular_component | synapse |
| B | 0050909 | biological_process | sensory perception of taste |
| B | 0051020 | molecular_function | GTPase binding |
| B | 0060041 | biological_process | retina development in camera-type eye |
| B | 0070062 | cellular_component | extracellular exosome |
| B | 0071380 | biological_process | cellular response to prostaglandin E stimulus |
| B | 0071870 | biological_process | cellular response to catecholamine stimulus |
| B | 0097381 | cellular_component | photoreceptor disc membrane |
| B | 1903561 | cellular_component | extracellular vesicle |
| G | 0005515 | molecular_function | protein binding |
| G | 0005834 | cellular_component | heterotrimeric G-protein complex |
| G | 0005886 | cellular_component | plasma membrane |
| G | 0007165 | biological_process | signal transduction |
| G | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| G | 0007191 | biological_process | adenylate cyclase-activating dopamine receptor signaling pathway |
| G | 0016020 | cellular_component | membrane |
| G | 0031681 | molecular_function | G-protein beta-subunit binding |
| G | 0045202 | cellular_component | synapse |
| G | 0048144 | biological_process | fibroblast proliferation |
| G | 0070062 | cellular_component | extracellular exosome |
| G | 0071380 | biological_process | cellular response to prostaglandin E stimulus |
| G | 0071870 | biological_process | cellular response to catecholamine stimulus |
| Q | 0004930 | molecular_function | G protein-coupled receptor activity |
| Q | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| Q | 0016020 | cellular_component | membrane |
| R | 0004930 | molecular_function | G protein-coupled receptor activity |
| R | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| R | 0016020 | cellular_component | membrane |
Functional Information from PROSITE/UniProt
| site_id | PS00678 |
| Number of Residues | 15 |
| Details | WD_REPEATS_1 Trp-Asp (WD) repeats signature. LVSAsqDgKLIIWDS |
| Chain | Residue | Details |
| B | LEU70-SER84 | |
| B | ILE157-ILE171 | |
| B | LEU285-ALA299 |
| site_id | PS00979 |
| Number of Residues | 19 |
| Details | G_PROTEIN_RECEP_F3_1 G-protein coupled receptors family 3 signature 1. VaNLLgLFyIPQVSyASSS |
| Chain | Residue | Details |
| R | VAL153-SER171 |
| site_id | PS00980 |
| Number of Residues | 25 |
| Details | G_PROTEIN_RECEP_F3_2 G-protein coupled receptors family 3 signature 2. CCFeCveCpdgeYsdet.DasACnkC |
| Chain | Residue | Details |
| R | CYS561-CYS585 |
| site_id | PS00981 |
| Number of Residues | 11 |
| Details | G_PROTEIN_RECEP_F3_3 G-protein coupled receptors family 3 signature 3. FNEAKfITFSM |
| Chain | Residue | Details |
| R | PHE801-MET811 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | MOD_RES: N-acetylalanine => ECO:0007744|PubMed:19413330, ECO:0007744|PubMed:22814378 |
| Chain | Residue | Details |
| G | ALA2 | |
| R | GLU671-GLN681 | |
| R | ALA746-SER769 | |
| R | TYR829-VAL836 | |
| Q | TYR20-PHE612 | |
| Q | GLU671-GLN681 | |
| Q | ALA746-SER769 | |
| Q | TYR829-VAL836 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | MOD_RES: Cysteine methyl ester => ECO:0000250|UniProtKB:P63212 |
| Chain | Residue | Details |
| G | CYS68 | |
| Q | GLY613-ILE635 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | LIPID: S-geranylgeranyl cysteine => ECO:0000250|UniProtKB:P63212 |
| Chain | Residue | Details |
| G | CYS68 | |
| R | ARG701-GLN724 | |
| R | LYS793-LYS805 | |
| Q | LYS636-GLU649 | |
| Q | ARG701-GLN724 | |
| Q | LYS793-LYS805 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | LIPID: S-palmitoyl cysteine => ECO:0000250|UniProtKB:P10824 |
| Chain | Residue | Details |
| A | CYS3 | |
| Q | LEU650-GLY670 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 3 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:P04896 |
| Chain | Residue | Details |
| A | ASP85 | |
| A | ASN144 | |
| A | ALA218 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:20068231 |
| Chain | Residue | Details |
| A | SER204 | |
| Q | PHE725-THR745 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) |
| Chain | Residue | Details |
| A | LYS152 | |
| Q | LEU770-PHE792 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 44 |
| Details | TRANSMEM: Helical; Name=6 => ECO:0000255 |
| Chain | Residue | Details |
| R | PHE806-THR828 | |
| Q | PHE806-THR828 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 50 |
| Details | TRANSMEM: Helical; Name=7 => ECO:0000255 |
| Chain | Residue | Details |
| R | GLU837-PHE862 | |
| Q | GLU837-PHE862 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 12 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:27434672, ECO:0007744|PDB:5K5S |
| Chain | Residue | Details |
| R | ARG66 | |
| Q | ASP234 | |
| Q | ARG415 | |
| Q | GLY557 | |
| R | THR145 | |
| R | GLU231 | |
| R | ASP234 | |
| R | ARG415 | |
| R | GLY557 | |
| Q | ARG66 | |
| Q | THR145 | |
| Q | GLU231 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:27434672, ECO:0000305|PubMed:27386547, ECO:0007744|PDB:5FBH, ECO:0007744|PDB:5FBK, ECO:0007744|PDB:5K5S |
| Chain | Residue | Details |
| R | ILE81 | |
| R | LEU87 | |
| R | LEU88 | |
| Q | ILE81 | |
| Q | LEU87 | |
| Q | LEU88 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:27434672, ECO:0000305|PubMed:27386547, ECO:0007744|PDB:5FBK, ECO:0007744|PDB:5K5S |
| Chain | Residue | Details |
| R | SER84 | |
| Q | SER84 |
| site_id | SWS_FT_FI13 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:27434672, ECO:0007744|PDB:5K5S, ECO:0007744|PDB:5K5T |
| Chain | Residue | Details |
| R | THR100 | |
| Q | THR100 |
| site_id | SWS_FT_FI14 |
| Number of Residues | 8 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:27386547, ECO:0000269|PubMed:27434672, ECO:0007744|PDB:5FBH, ECO:0007744|PDB:5FBK, ECO:0007744|PDB:5K5S |
| Chain | Residue | Details |
| R | SER147 | |
| R | ALA168 | |
| R | SER170 | |
| R | GLU297 | |
| Q | SER147 | |
| Q | ALA168 | |
| Q | SER170 | |
| Q | GLU297 |
| site_id | SWS_FT_FI15 |
| Number of Residues | 2 |
| Details | SITE: Important for ability of agonist AMG 416 to activate G-protein-coupled receptor activity => ECO:0000269|PubMed:26290606 |
| Chain | Residue | Details |
| R | CYS482 | |
| Q | CYS482 |
| site_id | SWS_FT_FI16 |
| Number of Residues | 8 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255 |
| Chain | Residue | Details |
| R | ASN90 | |
| R | ASN130 | |
| R | ASN386 | |
| R | ASN400 | |
| Q | ASN90 | |
| Q | ASN130 | |
| Q | ASN386 | |
| Q | ASN400 |
| site_id | SWS_FT_FI17 |
| Number of Residues | 14 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255, ECO:0000269|PubMed:27434672 |
| Chain | Residue | Details |
| R | ASN261 | |
| Q | ASN446 | |
| Q | ASN468 | |
| Q | ASN488 | |
| Q | ASN541 | |
| Q | ASN594 | |
| R | ASN287 | |
| R | ASN446 | |
| R | ASN468 | |
| R | ASN488 | |
| R | ASN541 | |
| R | ASN594 | |
| Q | ASN261 | |
| Q | ASN287 |
