9AX5
Cryo-EM structure of Phospholipase C epsilon PH-C terminus in complex with RhoA-GTP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 | polymer | 1468 | 165182.9 | 1 | UniProt (Q99P84) Pfam (PF00388) Pfam (PF00387) Pfam (PF00168) Pfam (PF00788) | Rattus norvegicus (Norway rat) | Phosphoinositide phospholipase C-epsilon-1,Phospholipase C-epsilon-1,PLC-epsilon-1 |
| 2 | B (C) | Transforming protein RhoA | polymer | 216 | 24569.1 | 1 | UniProt (P61586) Pfam (PF00071) | Homo sapiens (human) | Rho cDNA clone 12,h12 |
| 3 | C (A) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 4 | D (C) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) | |||
| 5 | E (C) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
A: 837 - 2281 (UniProt: Q99P84)
C: 2 - 193 (UniProt: P61586)
| PDB | External Database | Details |
|---|---|---|
| His 814 | - | expression tag |
| His 815 | - | expression tag |
| His 816 | - | expression tag |
| His 817 | - | expression tag |
| His 818 | - | expression tag |
| His 819 | - | expression tag |
| Ser 820 | - | expression tag |
| Ser 821 | - | expression tag |
| Gly 822 | - | expression tag |
| Val 823 | - | expression tag |
| Asp 824 | - | expression tag |
| Leu 825 | - | expression tag |
| Gly 826 | - | expression tag |
| Thr 827 | - | expression tag |
| Glu 828 | - | expression tag |
| Asn 829 | - | expression tag |
| Leu 830 | - | expression tag |
| Tyr 831 | - | expression tag |
| Phe 832 | - | expression tag |
| Gln 833 | - | expression tag |
| Ser 834 | - | expression tag |
| Asn 835 | - | expression tag |
| Ala 836 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| His -22 | - | expression tag |
| His -21 | - | expression tag |
| His -20 | - | expression tag |
| His -19 | - | expression tag |
| His -18 | - | expression tag |
| His -17 | - | expression tag |
| Asp -16 | - | expression tag |
| Tyr -15 | - | expression tag |
| Asp -14 | - | expression tag |
| Ile -13 | - | expression tag |
| Pro -12 | - | expression tag |
| Thr -11 | - | expression tag |
| Thr -10 | - | expression tag |
| Glu -9 | - | expression tag |
| Asn -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Tyr -6 | - | expression tag |
| Phe -5 | - | expression tag |
| Gln -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Met -1 | - | expression tag |
| Asp 0 | - | expression tag |
| Pro 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 189752.0 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 587.6 | |
| All* | Total formula weight | 190339.6 |
