8ZJE

Cryo-EM structure of kisspeptin receptor bound to TAK-448

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1,Guanine nucleotide-binding protein G(q) subunit alpha	polymer	353	41312.9	1	UniProt (P63096) UniProt (P50148) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein,Guanine nucleotide-binding protein alpha-q
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (R)	KiSS-1 receptor	polymer	398	42747.1	1	UniProt (Q969F8) Pfam (PF00001)	Homo sapiens (human)	KiSS-1R,G-protein coupled receptor 54,G-protein coupled receptor OT7T175,hOT7T175,Hypogonadotropin-1,Kisspeptins receptor,Metastin receptor
5	E (S)	scFv16	polymer	247	26277.3	1		Mus sp.
6	F (C)	ACE-DTY-HYP-ASN-THR-PHE-XZA-LEU-NMM-TRP-NH2	polymer	11	1208.3	1		synthetic construct

Sequence modifications

A: 1 - 28 (UniProt: P63096)

PDB	External Database	Details
Arg 29	-	linker
Ser 30	-	linker

A: 31 - 361 (UniProt: P50148)

PDB	External Database	Details
Ala 210	Gly 208	conflict
Ser 333	Ala 331	conflict

B: 7 - 345 (UniProt: P62873)

PDB	External Database	Details
Met -5	-	initiating methionine
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Gly 2	-	expression tag
Ser 3	-	expression tag
Leu 4	-	expression tag
Leu 5	-	expression tag
Gln 6	-	expression tag

R: 1 - 398 (UniProt: Q969F8)

PDB	External Database	Details
Trp 131	Ala 131	conflict

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	158151.2
All*	Total formula weight	158151.2

*Water molecules are not included.