8ZJE
Cryo-EM structure of kisspeptin receptor bound to TAK-448
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Guanine nucleotide-binding protein G(i) subunit alpha-1,Guanine nucleotide-binding protein G(q) subunit alpha | polymer | 353 | 41312.9 | 1 | UniProt (P63096) UniProt (P50148) Pfam (PF00503) In PDB | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein,Guanine nucleotide-binding protein alpha-q |
2 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 351 | 38744.4 | 1 | UniProt (P62873) Pfam (PF00400) In PDB | Homo sapiens (human) | Transducin beta chain 1 |
3 | G | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) In PDB | Homo sapiens (human) | G gamma-I |
4 | R | KiSS-1 receptor | polymer | 398 | 42747.1 | 1 | UniProt (Q969F8) Pfam (PF00001) In PDB | Homo sapiens (human) | KiSS-1R,G-protein coupled receptor 54,G-protein coupled receptor OT7T175,hOT7T175,Hypogonadotropin-1,Kisspeptins receptor,Metastin receptor |
5 | S | scFv16 | polymer | 247 | 26277.3 | 1 | Mus sp. | ||
6 | C | ACY-DTY-HYP-ASN-THR-PHE-XZA-LEU-NMM-TRP-NH2 | polymer | 11 | 1224.3 | 1 | synthetic construct |
Sequence modifications
A: 1 - 28 (UniProt: P63096)
A: 31 - 361 (UniProt: P50148)
B: 7 - 345 (UniProt: P62873)
R: 1 - 398 (UniProt: Q969F8)
PDB | External Database | Details |
---|---|---|
Arg 29 | - | linker |
Ser 30 | - | linker |
PDB | External Database | Details |
---|---|---|
Ala 210 | Gly 208 | conflict |
Ser 333 | Ala 331 | conflict |
PDB | External Database | Details |
---|---|---|
Met -5 | - | initiating methionine |
His -4 | - | expression tag |
His -3 | - | expression tag |
His -2 | - | expression tag |
His -1 | - | expression tag |
His 0 | - | expression tag |
His 1 | - | expression tag |
Gly 2 | - | expression tag |
Ser 3 | - | expression tag |
Leu 4 | - | expression tag |
Leu 5 | - | expression tag |
Gln 6 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Trp 131 | Ala 131 | conflict |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 158167.2 | |
All* | Total formula weight | 158167.2 |