8ZJD

Cryo-EM structure of kisspeptin receptor bound to KP-10

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	R	KiSS-1 receptor,KiSS-1 receptor,KiSS-1 receptor,KiSS-1 receptor,KiSS-1 receptor, G-protein coupled receptor 54, GPR54, KISS1R	polymer	512	55953.9	1	UniProt (Q969F8) In PDB	Homo sapiens (human)	KiSS-1R,G-protein coupled receptor 54,G-protein coupled receptor OT7T175,hOT7T175,Hypogonadotropin-1,Kisspeptins receptor,Metastin receptor
2	L	kisspeptin-10	polymer	11	1375.5	1		synthetic construct
3	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P62873) In PDB	Homo sapiens (human)	Transducin beta chain 1
4	S	scFv16	polymer	247	26277.3	1		Mus sp.
5	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) In PDB	Homo sapiens (human)	G gamma-I
6	A	Guanine nucleotide-binding protein G(i) subunit alpha-1,Guanine nucleotide-binding protein G(q) subunit alpha	polymer	353	41312.9	1	UniProt (P63096) UniProt (P50148) In PDB	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein,Guanine nucleotide-binding protein alpha-q

Sequence modifications

R: 2 - 355 (UniProt: Q969F8)

PDB	External Database	Details
Trp 131	Ala 131	conflict

R: 356 - 513 (PDB: 8ZJD)
B: 7 - 345 (UniProt: P62873)

PDB	External Database	Details
Met -5	-	initiating methionine
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Gly 2	-	expression tag
Ser 3	-	expression tag
Leu 4	-	expression tag
Leu 5	-	expression tag
Gln 6	-	expression tag

A: 1 - 28 (UniProt: P63096)

PDB	External Database	Details
Arg 29	-	linker
Ser 30	-	linker

A: 31 - 361 (UniProt: P50148)

PDB	External Database	Details
Ala 210	Gly 208	conflict
Ser 333	Ala 331	conflict

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	171525.2
All*	Total formula weight	171525.2

*Water molecules are not included.