8ZJD
Cryo-EM structure of kisspeptin receptor bound to KP-10
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (R) | KiSS-1 receptor,KiSS-1 receptor,KiSS-1 receptor,KiSS-1 receptor,KiSS-1 receptor, G-protein coupled receptor 54, GPR54, KISS1R | polymer | 512 | 55953.9 | 1 | UniProt (Q969F8) Pfam (PF00001) | Homo sapiens (human) | KiSS-1R,G-protein coupled receptor 54,G-protein coupled receptor OT7T175,hOT7T175,Hypogonadotropin-1,Kisspeptins receptor,Metastin receptor |
| 2 | B (L) | kisspeptin-10 | polymer | 11 | 1375.5 | 1 | UniProt (by SIFTS) (Q15726) | synthetic construct | |
| 3 | C (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 351 | 38744.4 | 1 | UniProt (P62873) Pfam (PF25391) | Homo sapiens (human) | Transducin beta chain 1 |
| 4 | D (S) | scFv16 | polymer | 247 | 26277.3 | 1 | Mus sp. | ||
| 5 | E (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 6 | F (A) | Guanine nucleotide-binding protein G(i) subunit alpha-1,Guanine nucleotide-binding protein G(q) subunit alpha | polymer | 353 | 41312.9 | 1 | UniProt (P63096) UniProt (P50148) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein,Guanine nucleotide-binding protein alpha-q |
Sequence modifications
R: 2 - 355 (UniProt: Q969F8)
R: 356 - 513 (PDB: 8ZJD)
B: 7 - 345 (UniProt: P62873)
A: 1 - 28 (UniProt: P63096)
A: 31 - 361 (UniProt: P50148)
| PDB | External Database | Details |
|---|---|---|
| Trp 131 | Ala 131 | conflict |
B: 7 - 345 (UniProt: P62873)
| PDB | External Database | Details |
|---|---|---|
| Met -5 | - | initiating methionine |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| His 0 | - | expression tag |
| His 1 | - | expression tag |
| Gly 2 | - | expression tag |
| Ser 3 | - | expression tag |
| Leu 4 | - | expression tag |
| Leu 5 | - | expression tag |
| Gln 6 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Arg 29 | - | linker |
| Ser 30 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Ala 210 | Gly 208 | conflict |
| Ser 333 | Ala 331 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 171525.2 | |
| All* | Total formula weight | 171525.2 |
