8ZEL

Crystal structure of human cytosolic beta-alanyl lysine dipeptidase Tyr314Phe mutant with a crystal soaked in beta-alanyl ornithine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Xaa-Arg dipeptidase	polymer	454	49732.8	1	UniProt (Q8IYS1) Pfam (PF01546) Pfam (PF07687)	Homo sapiens (human)	Beta-Ala-Lys dipeptidase
2	B, C (A)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
3	D (A)	L-ornithine	non-polymer		132.2	1	Chemie (ORN) PubChem (389) PubChem (6262) PubChem (71082)
4	E (A)	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS) PubChem (3777159)
5	F (A)	ACETIC ACID	non-polymer		60.1	1	Chemie (ACY)
6	G (A)	water	water		18.0	98	Chemie (HOH)

Sequence modifications

A: 20 - 436 (UniProt: Q8IYS1)

PDB	External Database	Details
Met -17	-	initiating methionine
Ala -16	-	expression tag
Ser -15	-	expression tag
Trp -14	-	expression tag
Ser -13	-	expression tag
His -12	-	expression tag
Pro -11	-	expression tag
Gln -10	-	expression tag
Phe -9	-	expression tag
Glu -8	-	expression tag
Gly -7	-	expression tag
Gly -6	-	expression tag
Ser -5	-	expression tag
Ser -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
His 2	-	expression tag
Ser 3	-	expression tag
Ser 4	-	expression tag
Gly 5	-	expression tag
Ser 6	-	expression tag
Gly 7	-	expression tag
Gly 8	-	expression tag
Gly 9	-	expression tag
Gly 10	-	expression tag
Gly 11	-	expression tag
Glu 12	-	expression tag
Asn 13	-	expression tag
Leu 14	-	expression tag
Tyr 15	-	expression tag
Phe 16	-	expression tag
Gln 17	-	expression tag
Gly 18	-	expression tag
Ser 19	-	expression tag
Phe 314	Tyr 314	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	49732.8
Non-Polymers*	Number of molecules	5
	Total formula weight	445.2
All*	Total formula weight	50178.0

*Water molecules are not included.