8ZBR

Cryo-EM structure of nanodisc-reconstituted wildtype human MRP4 (apo-form)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	ATP-binding cassette sub-family C member 4	polymer	1344	152203.5	1	UniProt (O15439) Pfam (PF00664) Pfam (PF00005)	Homo sapiens (human)	MRP/cMOAT-related ABC transporter,Multi-specific organic anion transporter B,MOAT-B,Multidrug resistance-associated protein 4
2	B, F, H (A)	CHOLESTEROL	non-polymer		386.7	3	Chemie (CLR)
3	C, D, E, G (A)	CHOLESTEROL HEMISUCCINATE	non-polymer		486.7	4	Chemie (Y01)

Sequence modifications

A: 1 - 1325 (UniProt: O15439)

PDB	External Database	Details
Met -10	-	initiating methionine
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Asp 1326	-	expression tag
Tyr 1327	-	expression tag
Lys 1328	-	expression tag
Asp 1329	-	expression tag
Asp 1330	-	expression tag
Asp 1331	-	expression tag
Asp 1332	-	expression tag
Lys 1333	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	152203.5
Non-Polymers*	Number of molecules	7
	Total formula weight	3106.9
All*	Total formula weight	155310.4

*Water molecules are not included.