8Z9O
Cryo-EM structure of human GPR4-Gs complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 338 | 37198.7 | 1 | UniProt (P54311) Pfam (PF00400) | Rattus norvegicus (Norway rat) | Transducin beta chain 1 |
| 2 | B (C) | Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 370 | 43234.9 | 1 | UniProt (P63092) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-stimulating G alpha protein |
| 3 | C (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 57 | 6261.2 | 1 | UniProt (P63212) Pfam (PF00631) | Bos taurus (domestic cattle) | G gamma-I |
| 4 | D (N) | Nanobody-35 | polymer | 126 | 13711.3 | 1 | synthetic construct | ||
| 5 | E (R) | G-protein coupled receptor 4 | polymer | 303 | 34657.5 | 1 | UniProt (P46093) Pfam (PF00001) | Homo sapiens (human) | G-protein coupled receptor 6C.l,GPR6C.l |
| 6 | F, G (R) | CHOLESTEROL | non-polymer | 386.7 | 2 | Chemie (CLR) | |||
| 7 | H (R) | water | water | 18.0 | 1 | Chemie (HOH) |
Sequence modifications
C: 11 - 394 (UniProt: P63092)
| PDB | External Database | Details |
|---|---|---|
| Ala 226 | Gly 212 | engineered mutation |
| Ala 268 | Glu 254 | engineered mutation |
| Lys 271 | Asn 257 | engineered mutation |
| Asp 274 | Lys 260 | engineered mutation |
| Lys 280 | Arg 266 | engineered mutation |
| Asp 284 | Thr 270 | engineered mutation |
| Thr 285 | Ile 271 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 135063.6 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 773.3 | |
| All* | Total formula weight | 135836.9 |
