8YY8

Fzd7 -Gs complex

Replaces:  7EVW

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	minGas	polymer	248	28907.7	1	Pfam (PF00503) UniProt (by SIFTS) (P63092)	Homo sapiens
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	340	37416.9	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	96	10366.8	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (N)	Nb35	polymer	149	16054.2	1		Homo sapiens
5	E (R)	Frizzled-7	polymer	586	66213.5	1	UniProt (O75084) Pfam (PF01392) Pfam (PF01534)	Homo sapiens (human)	Fz-7,hFz7,FzE3

Sequence modifications

C: 25 - 95 (UniProt: P59768)

PDB	External Database	Details
His 0	-	expression tag
His 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
Gly 6	-	expression tag
Gly 7	-	expression tag
Gly 8	-	expression tag
Ser 9	-	expression tag
Asp 10	-	expression tag
Ser 11	-	expression tag
Leu 12	-	expression tag
Glu 13	-	expression tag
Phe 14	-	expression tag
Ile 15	-	expression tag
Ala 16	-	expression tag
Ser 17	-	expression tag
Lys 18	-	expression tag
Leu 19	-	expression tag
Ala 20	-	expression tag
Gly 21	-	expression tag
Gly 22	-	expression tag
Gly 23	-	expression tag
Ser 24	-	expression tag
Ser 92	Cys 68	engineered mutation

R: 38 - 574 (UniProt: O75084)

PDB	External Database	Details
Met 14	-	initiating methionine
Lys 15	-	expression tag
Thr 16	-	expression tag
Ile 17	-	expression tag
Ile 18	-	expression tag
Ala 19	-	expression tag
Leu 20	-	expression tag
Ser 21	-	expression tag
Tyr 22	-	expression tag
Ile 23	-	expression tag
Phe 24	-	expression tag
Cys 25	-	expression tag
Leu 26	-	expression tag
Val 27	-	expression tag
Phe 28	-	expression tag
Ala 29	-	expression tag
Asp 30	-	expression tag
Tyr 31	-	expression tag
Lys 32	-	expression tag
Asp 33	-	expression tag
Asp 34	-	expression tag
Asp 35	-	expression tag
Asp 36	-	expression tag
Lys 37	-	expression tag
His 575	-	expression tag
His 576	-	expression tag
His 577	-	expression tag
His 578	-	expression tag
His 579	-	expression tag
His 580	-	expression tag
His 581	-	expression tag
His 582	-	expression tag
His 583	-	expression tag
His 584	-	expression tag
Gly 585	-	expression tag
Leu 586	-	expression tag
Asn 587	-	expression tag
Asp 588	-	expression tag
Ile 589	-	expression tag
Phe 590	-	expression tag
Glu 591	-	expression tag
Ala 592	-	expression tag
Gln 593	-	expression tag
Lys 594	-	expression tag
Ile 595	-	expression tag
Glu 596	-	expression tag
Trp 597	-	expression tag
His 598	-	expression tag
Glu 599	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	158959.1
All*	Total formula weight	158959.1

*Water molecules are not included.