8Y8B
Local structure of HCoV-HKU1C spike in complex with TMPRSS2 and glycan
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | Spike glycoprotein | polymer | 1263 | 140242.5 | 2 | UniProt (Q0ZME7) Pfam (PF16451) Pfam (PF09408) Pfam (PF19209) Pfam (PF01601) In PDB | Human coronavirus HKU1 (isolate N5) | S glycoprotein,E2,Peplomer protein |
| 2 | C, T | Transmembrane protease serine 2 | polymer | 383 | 42283.6 | 2 | UniProt (O15393) Pfam (PF15494) Pfam (PF00089) In PDB | Homo sapiens (human) | Serine protease 10 |
| 3 | D | alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 748.7 | 1 | In PDB GlyTouCan (G81315DD) | |||
| 4 | E, F | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 2 | In PDB GlyTouCan (G15407YE) | |||
| 5 | G, H, I, J, K | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 5 | In PDB GlyTouCan (G42666HT) | |||
| 6 | A | methyl 9-O-acetyl-5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosidonic acid | non-polymer | 365.3 | 1 | Chemie (MJJ) | |||
| 7 | A, B | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 5 | Chemie (NAG) |
Sequence modifications
A, B: 14 - 1276 (UniProt: Q0ZME7)
C, T: 109 - 491 (UniProt: O15393)
| PDB | External Database | Details |
|---|---|---|
| Gly 752 | Arg 752 | engineered mutation |
| Gly 753 | Arg 753 | engineered mutation |
| Ser 754 | Lys 754 | engineered mutation |
| Gly 755 | Arg 755 | engineered mutation |
| Ser 756 | Arg 756 | engineered mutation |
| Pro 902 | Leu 902 | engineered mutation |
| Pro 980 | Ser 980 | engineered mutation |
| Pro 1023 | Asn 1023 | engineered mutation |
| Pro 1067 | Asn 1067 | engineered mutation |
| Pro 1068 | Leu 1068 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Asp 250 | Ser 250 | engineered mutation |
| Asp 251 | Ser 251 | engineered mutation |
| - | Arg 252 | deletion |
| Asp 252 | Gln 253 | engineered mutation |
| Asp 253 | Ser 254 | engineered mutation |
| Lys 254 | Arg 255 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 365052.2 | |
| Branched | Number of molecules | 8 |
| Total formula weight | 4043.8 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1471.4 | |
| All* | Total formula weight | 370567.3 |
